文献类型：期刊文献

中文题名：蒙古栎天然群体遗传多样性的AFLP分析

英文题名：AFLP ANALYSIS ON GENETIC DIVERSITY OF QUERCUS MONGOLICA POPULATIONS

作者：李文英[1] 顾万春[2] 周世良[3]

第一作者：李文英

机构：[1]中国林业科学研究院科技信息研究所;[2]中国林业科学研究院林业研究所;[3]中国科学院植物研究所系统与进化植物学开放研究实验室

年份：2003

卷号：39

期号：5

起止页码：29-36

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家"十五"科技攻关"林木种质资源保存技术创新与利用研究"(项目编号 :2 0 0 1BA5 11B10 );国家重大基础性工作项目"林木种质资源收集与保存"(项目编号 :科技部 2 0 0 1年 13号 )的部分内容

语种：中文

中文关键词：蒙古栎;天然群体;遗传多样性;AFLP分析;Ant分析;多态带;群体特异带;遗传差异;遗传分化系数

外文关键词：Quercus mongolica, Quercus liaotungensis , Population, AFLP marker, Genetic diversity

分类号：S792.18

摘要：以黑龙江省小兴安岭嘉荫、内蒙古大青沟和河北省雾灵山的 3个蒙古栎群体及北京东灵山辽东栎群体为供试材料 ,用筛选出的 4对荧光引物 ,对 4个群体共计 96个个体进行AFLP分析 ,每对AFLP引物扩增出 6 3～ 1 1 3条 ,共得到 346条多态带 ;群体特异带及群体间共有带的差异与分布揭示了群体间的遗传差异及相似性 ;蒙古栎遗传多样性主要存在于群体内 ,群体间的遗传分化系数Gst为 0 0 77。蒙古栎在种级水平的遗传多样性参数略高于群体水平 ,多态带百分率P分别为 96 8%、6 7 2 % ,有效等位基因数Ae分别为 1 2 2 0、1 2 0 8,Nei基因多样性指数H分别为 0 1 4 5、0 1 34,Shannon多样性指数I分别为 0 2 4 6、0 2 0 8。东灵山辽东栎群体的P为 6 7 6 % ,Shannon多样性指数I为 0 2 2 0 ,Nei基因多样性指数H为 0 1 34。UPGMA聚类分析结果表明 ,蒙古栎自然群体间的遗传距离有随地理距离跨度增加而递增的趋势。蒙古栎遗传多样性偏低可能与其在历史上长期用作经济树种、人为干预和环境破坏较为严重。
Three Quercus mongolica populations located at Jiayin, Xiaoxing′anling Mountain in Heilongjiang Province, Daqinggou in Inner Mongolia Autonomous Region, and Wulingshan in Hebei Province, and one Q. liaotungensis (a variety of Q. mongolica ) population at Donglingshan in Beijing, totally 96 individuals were selected and analyzed by amplifications using 4 pairs of AFLP primers screened, each primer pair produced 63～113 bands and 346 polymorphic bands were obtained.As for Q.mongolica at species level, the effective number of alleles per locus ( Ae ) was 1 220, the percentage of polymorphic band ( P ) was 96 8%, and the Nei gene diversity index ( H ) was 0 145, Shannon's information index ( I ) was 0 246. At population level, the estimates were Ae=1 208,P =67 2%, and H=0 134,I=0 208 . In contrast, the estimates for Q.liaotungensis were almost the same ( Ae=1 5,P =67 6%, H=0 134, I=0 220 ). As for Q.mongolica , genetic differentiation coefficient among populations ( Gst ) was 0 077. According to the UPGMA cluster analysis, the genetic distance among Q.mongolica populations became larger with the increase of geographical distance. The low level of genetic diversity of Q.mongolica might relate to the long term utilization as economic tree species in history, comparatively serious disturbance and environmental damage by human beings, and secondary forests of the existed stands.