文献类型：期刊文献

英文题名：Genomic insights into drought adaptation of the forage shrub Caragana korshinskii (Fabaceae) widely planted in drylands

作者：Mei, Fengyuan[1] Yang, Tianrui[2] Chao, Haoyu[3] Ma, Xiaohui[1] Wu, Jingjing[1] Yang, Qi[2] Ren, Guangpeng[1] Song, Li[2] Wang, Qian[2] Qi, Liwang[4] Fu, Xinxing[5] Gao, Cuiping[7] Wang, Ruigang[2,8] Chen, Ming[3] Fang, Xiangwen[1] Liu, Jianquan[1] Li, Guojing[2,6,7] Wu, Shengdan[1]

第一作者：Mei, Fengyuan

通信作者：Liu, JQ[1];Wu, SD[1];Li, GJ[2];Li, GJ[3];Li, GJ[4]

机构：[1]Lanzhou Univ, Coll Ecol, State Key Lab Herbage Improvement & Grassland Agro, Lanzhou 730000, Peoples R China;[2]Inner Mongolia Agr Univ, Inner Mongolia Key Lab Plants Advers Adaptat & Gen, Hohhot 010018, Peoples R China;[3]Zhejiang Univ, Coll Life Sci, Dept Bioinformat, Hangzhou 310058, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[5]Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China;[6]Inner Mongolia Agr Univ, Key Lab Forage Cultivat Proc & High Efficient Util, Minist Agr, Hohhot 010021, Peoples R China;[7]Inner Mongolia Agr Univ, Key Lab Grassland Resources, Minist Educ, Hohhot 010021, Peoples R China;[8]Mengshu Ecol Construct Grp Co Ltd, Inner Mongolia Enterprise Key Lab Tree Breeding, Hohhot 011517, Peoples R China

年份：2025

卷号：121

期号：3

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20250617833634);Scopus(收录号：2-s2.0-85217040825);WOS:【SCI-EXPANDED(收录号:WOS:001414085700001)】；

基金：This research was partly supported by the National Natural Science Foundation of China-Gansu Joint Fund (U22A20452), the National Natural Science Foundation of China (32260372, 32471686, 32100170, 32260055, and 32325036), the Major Project of Inner Mongolia Natural Science Foundation (2024ZD17 and 2019ZD05), the Scientific and Technological Projects of Inner Mongolia (2023KJHZ0013 and 2023KYPT0016), and the University Scientific and Technological Innovation Team Project of Inner Mongolia (BR22-13-05 and NMGIRT2222), the Fundamental Research Funds for the Central Universities (lzujbky-2021-ct14), and the Gansu Science and Technology Major Project (22ZD6FA052). We received computational support from Supercomputing Center of Lanzhou University.

语种：英文

外文关键词：Caragana korshinskii; drought adaptation; tandem duplication; dehydrin; forage shrub; drylands

摘要：The Korshinsk peashrub (Caragana korshinskii), known for its exceptional drought tolerance, is widely cultivated in arid and semi-arid regions for vegetation restoration and as a vital forage plant. To elucidate the genomic basis of its drought tolerance, we generated a chromosomal-scale genome sequence of C. korshinskii. Our synteny analysis disputes the previously hypothesized genus-specific whole-genome duplication event, as suggested by earlier transcriptome study of this species and its congeners. We identified that tandem duplications were critical for the expansion of gene families, such as early light-induced protein, heat shock protein 100, and Dehydrin, which are involved in cellular protection processes. These expansions are likely pivotal to the superior drought tolerance observed in C. korshinskii, as evidenced by the elevated gene expression of these genes under drought conditions. Furthermore, overexpression studies of seven tandemly duplicated DHN genes revealed a substantial enhancement in drought survival rates of seedlings, likely attributable to increased gene dosage effects. Conversely, gene silencing via virus-induced gene silencing demonstrated opposing effects. Additionally, we have established the CakorDB, a genomic resource database for C. korshinskii (), accessible freely to the scientific community. Collectively, our study not only provides a valuable genomic resource for the Korshinsk peashrub but also highlights the genetic adaptations that enable C. korshinskii to thrive in desert environments, positioning its stress-responsive genes as a valuable genetic reservoir for breeding drought-resistant crops.