文献类型：期刊文献

中文题名：不同氮素供给水平对84K杨幼苗碳氮代谢的影响

英文题名：Effects of Nitrogen Supply Levels on Carbon and Nitrogen Metabolism of Populus alba × P. glandulosa Seedlings

作者：刘奇峰[1] 李卓蓉[1] 吴江婷[1] 罗志斌[1]

第一作者：刘奇峰

机构：[1]中国林业科学研究院林业研究所

年份：2019

卷号：32

期号：6

起止页码：63-72

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：树木生长适应环境的分子生理基础(2016YFD0600105)

语种：中文

中文关键词：氮素;84K杨;碳氮代谢;氨基酸;蔗糖;碳氮比

外文关键词：nitrogen;Populus alba×P.glandulosa;carbon and nitrogen metabolism;amino acid;sucrose;carbon nitrogen ratio.

分类号：S718.43

摘要：[目的]解析不同氮素供给水平下杨树幼苗的生长与碳氮代谢特征,对提高木材产量和品质以及科学制定人工林养分管理策略具有重要意义。[方法]分别以高氮、正常氮和低氮供给处理84K杨(Populus alba×P.glandulosa),分析其生长特征与碳氮代谢变化特征。[结果]高氮处理增加了净光合速率、根中的蔗糖含量以及叶中的淀粉含量和生物量,促进84K杨的碳代谢;高氮处理促进了84K杨根中NH4^+和NO3^-吸收和积累,提高了谷氨酸合成酶的活性,增加了大多数氨基酸含量以及总氮含量,促进了84K杨的氮代谢。低氮处理降低了净光合速率、叶中的大多数可溶性糖含量和淀粉含量,抑制84K杨的碳代谢;低氮处理会降低植物体内NH4^+、NO3^-含量,降低根中的硝酸还原酶(NR)的活性,降低氨基酸含量以及总氮含量,抑制84K杨的氮代谢。[结论]这些结果表明84K杨中的碳代谢与氮代谢响应环境中的氮素的盈亏变化表现出很高的一致性,即外界环境中氮素充足时,碳代谢随着氮代谢的增加而增加;而外界环境中氮素亏缺时,碳代谢随着氮代谢的降低而降低。
[Objective]To investigate the carbon and nitrogen metabolism of Populus seedlings under different nitrogen levels.[Method]Populus alba×P.glandulosa seedlings were treated with high(16 mmol/L N),normal(4 mmol/L N)and low(1 mmol/L N)nitrogen levels,respectively.The growth,carbon and nitrogen metabolism of Populus alba×P.glandulosa seedlings under the three nitrogen supply levels were analyzed.[Result]High nitrogen led to increased net photosynthetic rate,sucrose content in root,starch content and biomass in the leaves,promoted the carbon metabolism in P.alba×P.glandulosa.Meanwhile,the absorption and accumulation of NH4^+and NO3^-in the roots,GOGAT activities in the roots and leaves,the amino acid contents and total N contents were elevated under high nitrogen levels compared with that under normal nitrogen supply,which resulted in enhanced nitrogen metabolism in P.alba×P.glandulosa.In contrast,low nitrogen treatment brought about reduced net photosynthetic rate and the contents of most soluble sugars and starch in leaves,inhibited the carbon metabolism in P.alba×P.glandulosa.Additionally,low nitrogen supply also induced the decreases of NH4^+and NO3^-contents in the roots and leaves,decreased the nitrate reductase activities in the roots,and the contents of amino acid and total N in the roots and leaves,leading to inhibited nitrogen metabolism in P.alba×P.glandulosa.[Conclusion]The carbon metabolism of P.alba×P.glandulosa is highly linked with nitrogen metabolism in response to changing nitrogen levels in the environments.Carbon and nitrogen metabolism is enhanced when nitrogen supply is abundant,and both are inhibited under nitrogen deficiency condition.