文献类型：期刊文献

英文题名：Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology

作者：Hou, Chen[1,2] Tian, Yuxin[3,4] Wang, Yingli[1,2] Lian, Huiming[1,2] Liang, Dongcheng[1,2] Shi, Shengqing[5] Deng, Nan[3,4] He, Boxiang[1,2]

第一作者：Hou, Chen

通信作者：He, BX[1];He, BX[2];Deng, N[3];Deng, N[4]

机构：[1]Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Uti, Guangzhou 510520, Peoples R China;[2]Guangdong Acad Forestry, Guangshanyilu 233, Guangzhou 510520, Peoples R China;[3]Hunan Acad Forestry, 658 Shaoshan Rd, Changsha 410004, Hunan, Peoples R China;[4]Hunan Cili Forest Ecosyst State Res Stn, Changsha 410004, Hunan, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2021

卷号：11

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000658858700012)】；

基金：We thank Guan-Shen Liu (Biomarker Technologies, Inc., Beijing, China) for providing valuable technical and analytical assistance. We acknowledge TopEdit LLC for the linguistic editing and proofreading during the preparation of this manuscript. We also thank the three anonymous viewers for their constructive suggestions and comments. This work was supported by the Natural Science Foundation of Guangdong Province, China (No. 2018A0303130163 to C.H.).

语种：英文

摘要：Gnetum is a pantropical distributed gymnosperm genus. As being dioecious, Gnetum species apply female and male strobili to attract and provide nutrition to insect pollinators. Due to its unique gross morphology, a Gnetum male strobilus receives much attention in previous taxonomic and evolutionary studies. However, underlying molecular mechanisms that control male strobilus development and pollination adaptation have not been well studied. In the present study, nine full-length transcriptomes were sequenced from three developmental stages of the G. luofuense male strobili using Oxford Nanopore Technologies. In addition, weighted gene co-expression network analysis (WGCNA), and RT-qPCR analysis were performed. Our results show that a total of 3138 transcription factors and 466 long non-coding RNAs (lncRNAs) were identified, and differentially expressed lncRNAs and TFs reveal a dynamic pattern during the male strobilus development. Our results show that MADS-box and Aux/IAA TFs were differentially expressed at the three developmental stages, suggesting their important roles in the regulation of male strobilus development of G. luofuense. Results of WGCNA analysis and annotation of differentially expressed transcripts corroborate that the male strobilus development of G. luofuense is closely linked to plant hormone changes, photosynthesis, pollination drop secretion and reproductive organ defense. Our results provide a valuable resource for understanding the molecular mechanisms that drive organ evolution and pollination biology in Gnetum.