文献类型：期刊文献

英文题名：Transcripts and ABA-dependent signaling in response to drought stress in Hippophae rhamnoides L

作者：Zhang, Tong[1] Gao, Guori[1] Liu, Juanjuan[1] Yang, Guijuan[1] Lv, Zhongrui[1] Zhang, Jianguo[1,2] He, Caiyun[1]

通信作者：Zhang, JG[1];He, CY[1];Zhang, JG[2]|[a0005b245645e47e67b16]何彩云;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Peoples R China

年份：2020

卷号：34

期号：4

起止页码：1033-1045

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000529838200002)】；

基金：We would like to thank Mrs. H.M. Luo for her assistance with sample collection. We would also like to thank everyone who contributed to this manuscript in various capacities. This work was supported by the Beijing Natural Science Foundation [No. 6192027].

语种：英文

外文关键词：Sea buckthorn; RNA sequencing; Drought; ABA-dependent signaling; Flavonoids biosynthesis

摘要：Key message This study revealed the ABA-dependent signaling pathway and flavonoid synthesis pathway of sea buckthorn under drought conditions from plant physiology and gene expression levels. As climate changes and temperatures rise, sea buckthorn (Hippophae rhamnoides L.) is increasingly exposed to numerous challenges, particularly aridity. In this study, the leaves of sea buckthorn were harvested from plants in drought-recovery cycles and selected for RNA sequencing to uncover the underlying genetic basis of drought stress response through physiological and biochemical analyses. The results revealed that drought conditions significantly (p < 0.05) decreased leaf water potential, net photosynthetic rate, and stomatal conductance, Abscisic acid (ABA) and flavone content significantly (p < 0.05) increased under drought conditions and recovered after rehydration. Compared with the control and recovery irrigation conditions, respectively, 7734 and 7338 genes were differentially expressed under drought stress. Major differentially expressed genes involved in ABA-dependent signaling and flavonoid biosynthesis pathways were identified. Six hub genes of the co-expression network were blasted against the Drought Stress Gene Database and were found to have direct relationships with 25 genes there were related to cellular responses to stimuli. Five hub genes and seven drought transcription factors were validated by RT-qPCR. This study elucidated the molecular mechanism of the ABA-dependent signaling pathway that affects stomatal closure and flavonoid biosynthesis in antioxidant systems under drought conditions in sea buckthorn. The identified six hub genes will serve as a valuable resource and provide essential information for improving sea buckthorn tolerance to drought conditions.