文献类型：期刊文献

英文题名：Comprehensive Analysis and Functional Verification of the Pinus massoniana NBS-LRR Gene Family Involved in the Resistance to Bursaphelenchus xylophilus

作者：Xie, Yini[1,2,3] Liu, Bin[1,3] Gao, Kai[1,3] Zhao, Yunxiao[1] Li, Wenhua[1,3] Deng, Lili[1,3] Zhou, Zhichun[1,3] Liu, Qinghua[1,3]

第一作者：Xie, Yini

通信作者：Zhou, ZC[1];Liu, QH[1];Zhou, ZC[2];Liu, QH[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Nanjing Forestry Univ, Fac Forestry, Nanjing 210037, Peoples R China;[3]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Peoples R China

年份：2023

卷号：24

期号：3

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000932901900001)】；

基金：This work was supported by the Zhejiang Science and Technology Program (2020C02007), the National Natural Science Foundation of China (31470670), the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02070-5-2), and the Forestry Science and Technology Innovation Special Project of Jiangxi Forestry Bureau (2021.No.13).

语种：英文

外文关键词：NBS-LRR gene family; disease resistance genes; Pinus massoniana; pine wood nematode; PmNBS-LRR97; ROS

摘要：Pinus massoniana Lamb. is a crucial timber and resin conifer in China, but its plantation industry is threatened by outbreaks of pine wilt disease (PWD) caused by Bursaphelenchus xylophilus (pinewood nematode; PWN). However, as of yet, there is no comprehensive analysis of NBS-LRR genes in P. massoniana involved in its defense against PWN. In this study, 507 NBS genes were identified in the transcriptome of resistant and susceptible P. masoniana inoculated with the PWN. The phylogenetic analysis and expression profiles of resistant and susceptible P. massoniana revealed that the up-regulated PmNBS-LRR97 gene was involved in conferring resistance to PWN. The results of real-time quantitative PCR (qRT-PCR) showed that PmNBS-LRR97 was significantly up-regulated after PWN infection, especially in the stems. Subcellular localization indicated that PmNBS-LRR97 located to the cell membrane. PmNBS-LRR97 significantly activated the expression of reactive oxygen species (ROS)-related genes in P. massoniana. In addition, the overexpression of PmNBS-LRR97 was capable of promoting the production of ROS, aiding in plant growth and development. In summary, PmNBS-LRR97 participates in the defense response to PWN and plays an active role in conferring resistance in P. massoniana. This finding provides new insight into the regulatory mechanism of the R gene in P. massoniana.