文献类型：期刊文献

中文题名：青藏铁路沿线土壤可培养微生物种群多样性分析

英文题名：Analysis of Microbial Diversity in Soil along the Qinghai-Tibet Railway

作者：刘振静[1] 李潞滨[1] 庄彩云[1] 周金星[1] 杨凯[2] 韩继刚[3]

第一作者：刘振静

机构：[1]中国林业科学研究院林业研究所、国家林业局林木培育实验室,北京100091;[2]北京农学院农业应用新技术北京市重点实验室,北京102206;[3]河北大学生命科学学院,河北保定071002

年份：2008

卷号：21

期号：6

起止页码：176-181

中文期刊名：环境科学研究

外文期刊名：Research of Environmental Sciences

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：铁道部重大科技计划项目(2004G009);河北大学博士启动经费资助项目

语种：中文

中文关键词：青藏铁路;可培养微生物;种群多样性

外文关键词：Qinghai-Tibet railway; culturable microorganism; population diversity

分类号：X53

摘要：选择青藏铁路沿线不同海拔高度的10个地点采集土壤样品,采用3种不同的培养基分离培养其中的微生物;提取各土壤样品可培养微生物菌体基因组DNA,进行16S rDNA的PCR扩增,对可培养微生物种群多样性进行分析.结果表明,该地区可培养微生物的数量为1×106～8×106CFU/g.16SrDNA序列分析发现,分离培养的微生物主要包括厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)和变形菌门γ亚群(Gammaproteobacteria)3个类群,分别占菌株总数的53.1%,29.6%和16.0%.其中,Bacillussp.为优势菌属,占菌株总数的29.6%;Bacillussimplex为优势种,占菌株总数的12.3%.
Ten soil samples were collected at different altitudes along the Qinghai-Tibet railway. Three kinds of medium were used to isolate microorganisms, whose genome DNA was extracted for PCR amplification of 16S rDNA, to analyze microbial diversity of different soil samples. The results showed that the amount of culturable microbes ranged from 1 × 10^6 to 8× 10^6 CFU/g. Analysis based on 16S rDNA indicated that there were Firmicutes, Actinobaeteria and Gammaproteobacteria, which can be cultured, and accounted for 53.1% , 29.6%, 16.0% of total isolated strains, respectively. Bacillus sp. was the predominant genus and accounted for 29.6 % of total isolated strains, while Bacillus simplex was the predominant species in this area and accounted for 12.3 % of total isolated strains.