文献类型：期刊文献

英文题名：Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance

作者：Yao, Jun[1] Shen, Zedan[1,2] Zhang, Yanli[1] Wu, Xia[1] Wang, Jianhui[3] Sa, Gang[1,4] Zhang, Yuhong[1,5] Zhang, Huilong[1] Deng, Chen[1] Liu, Jian[1] Hou, Siyuan[1] Zhang, Ying[1] Zhang, Yinan[1,6] Zhao, Nan[1] Deng, Shurong[1,5] Lin, Shanzhi[1] Zhao, Rui[1] Chen, Shaoliang[1]

第一作者：Yao, Jun

通信作者：Zhao, R[1];Chen, SL[1]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Coll Biol Sci & Technol, Box 162, Beijing 100083, Peoples R China;[2]Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Peoples R China;[3]Bluescape Hebei Biotech Co Ltd, Baoding 071000, Peoples R China;[4]Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[6]Chinese Acad Forestry, Forestry Inst New Technol, Beijing 100091, Peoples R China

年份：2020

卷号：71

期号：4

起止页码：1527-1539

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000518530400028)】；

基金：The research was supported jointly by the National Natural Science Foundation of China (grant nos 31770643 and 31570587),Beijing Natural Science Foundation (grant nos 6182030 and 6172024), the Fundamental Research Funds for the Central Universities (grant no. 2019ZY25), the Program of Introducing Talents of Discipline to Universities (111 Project, grant no. B13007), and the Beijing Advanced Innovation Center for Tree Breeding by Molecular Design (Beijing Forestry University). Prof. Dr Yule Liu (School of Life Sciences, Tsinghua University) is sincerely acknowledged for providing TRV vectors.

语种：英文

外文关键词：DNA affinity purification sequencing; electrophoretic mobility shift assay; H+-ATPase; luciferase reporter assay; NaCl; non-invasive microtest technique; PeWRKY1; virus-induced gene silencing; W-box

摘要：Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that beta-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.