文献类型：期刊文献

英文题名：Genome-wide characterization of the SPL gene family involved in the age development of Jatropha curcas

作者：Yu, Niu[1] Yang, Jin-Chang[1] Yin, Guang-Tian[1] Li, Rong-Sheng[1] Zou, Wen-Tao[1]

第一作者：余纽

通信作者：Yu, N[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry Adm Trop Forestry Res, 682 Guang Shan Yi Rd, Guangzhou 510520, Peoples R China

年份：2020

卷号：21

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000537084200001)】；

基金：This study was financially supported by National Natural Science Foundation of China (grant Number 31600538) and Fundamental Research Funds of Chinese Academy of Forestry (grant Number CAFYBB2018QB003 and CAFYBB2017SY023). The funding body initially approved the project and played no role in the design of the study or collection, analysis and interpretation of data.

语种：英文

外文关键词：Jatropha curcas; SPL; Genome-wide; miR156; Expression patterns; Age development

摘要：BackgroundSPL (SQUAMOSA-promoter binding protein-like) proteins form a large family of plant-specific transcription factors that play essential roles in various aspects of plant growth and development. They are potentially important candidates for genetic improvement of agronomic traits. However, there were limited information about the SPL genes in Jatropha curcas, an important biofuel plant.ResultsIn Jatropha, 15 JcSPL genes were identified. Phylogenetic analysis revealed that most of the JcSPLs were closely related to SPLs from woody plant rather than herbaceous plant and distantly related to monocotyledon SPLs. Gene structure, conserved motif and repetitive sequence analysis indicated diverse and specific functions of some JcSPL genes. By combination of target prediction and degradome sequencing analysis, 10 of the 15 JcSPLs were shown to be targets of JcmiR156. Quantitative PCR analysis showed diversified spatial-temporal expression patterns of JcSPLs. It is interesting that the expression levels of JcSPL3 were the highest in all tissues examined in 7- or 10-year-old plants and exhibited increasing trend with plant age, suggesting its important role in the regulation of age development in Jatropha. Overexpression of JcSPL3 in Arabidopsis resulted in earlier flowering time, shorter silique length and reduced biomass of roots.ConclusionsThrough comprehensive and systematic analysis of phylogenetic relationships, conserved motifs, gene structures, chromosomal locations, repetitive sequence and expression patterns, 15 JcSPL genes were identified in Jatropha and characterized in great detail. These results provide deep insight into the evolutionary origin and biological significance of plant SPLs and lay the foundation for further functional characterization of JcSPLs with the purpose of genetic improvement in Jatropha.