文献类型：期刊文献

中文题名：云南金钱槭天然居群等位酶遗传多样性研究

英文题名：Allozyme-based Genetic Diversity of Dipteronia dyeriana in China

作者：欧阳志勤[1] 王兵益[2] 党晓栋[3] 张莘[3] 丁开宇[3]

第一作者：欧阳志勤

机构：[1]云南省环境科学研究院,昆明650034;[2]中国林业科学研究院资源昆虫研究所,昆明650224;[3]云南大学生命科学学院,昆明650091

年份：2009

期号：5

起止页码：461-466

中文期刊名：武汉植物学研究

外文期刊名：Journal of Wuhan Botanical Research

收录：北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：云南省自然科学基金资助项目(2002C0081M)

语种：中文

中文关键词：云南金钱槭;等位酶;遗传多样性;生境片段化;保育策略

外文关键词：Dipteronia dyeriana Henry ; Allozyme ; Genetic diversity ; Habitat fragmentation ; Conservation strategy

分类号：Q943

摘要：云南金钱槭（Dipteronia dyeriana）是我国特有的国家Ⅱ级重点保护珍稀濒危植物,仅分布于云南的文山、屏边和蒙自三县交界地区。采用水平切片淀粉凝胶电泳等位酶分析方法,对采自三个县的天然居群的63个样品进行了遗传多样性检测。8个酶系统的13个等位基因位点分析表明,云南金钱槭的遗传多样性水平低,其平均多态位点百分率P=15.4%,每位点平均等位基因数A=1.2,平均预期杂合度He=0.064,各居群都偏离Hardy-Weinberg平衡,杂合子过量,居群间的基因分化系数GST=0.099,遗传变异主要发生在居群内（90.1%）,居群间分化较小,居群间遗传一致度较高（I=0.985～1）,具有一定的基因流（Nm=2.669）。不加权对平均法（UPGMA）聚类可将4个居群分为两支,水头箐居群和马家寨居群亲缘关系最近,聚类后与黑洞居群形成一支再和中槽子居群聚类,这与其地理分布格局大致吻合。据此提出了关于云南金钱槭保育策略的建议。
Dipteronia dyeriana Henry is a rare and endangered species restricted in a small adjacent corner of Wenshan,Pingbian and Mengzi counties of Yunnan, southwest China. The genetic diversity of four natural populations was estimated using allozyme markers. Thirteen loci of eight enzyme systems were scored for the analysis of population genetic diversity and structure. A low genetic diversity was observed in this rare species ,with a mean percentage of polymorphic loci P = 15.4% ,an average number of alleles per locus A = 1.2, and an average of expected heterozygosity He = 0. 064. All populations deviated signifi- cantly from the Hardy-Weinberg equilibrium. Very slight population differentiation was detected （GST = 0. 099）. Nei＇ s genetic identity ranged from 0. 985 to 1. Moderate gene flow was inferred （Nm = 2. 669）. According to the Unweighted Pair Group Method with Arithmetic （UPGMA）cluster analysis based on the genetic distance, Mengzi and Majiazhai populations are clustered in one clade and its genetic distances are positively related to the geographic disance. For conservation purpose, we strongly suggest strict protection of the natural populations of the species should be conducted,and ex situ conservation could be an effective supplement.