文献类型：期刊文献

英文题名：A male- and female-sterile mutant of Kentucky bluegrass (Poa pratensis L.) induced by space radiation

作者：Han, Lei[1] Luo, Dong[1,2] Li, Deying[] Qian, Yongqiang[1] Sun, Zhenyuan[1]

第一作者：Han, Lei

通信作者：Sun, ZY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding Forest Cultivat, State Forestry Adm, Beijing 100091, Peoples R China;[2]N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA

年份：2011

卷号：131

期号：1

起止页码：67-73

外文期刊名：SCIENTIA HORTICULTURAE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298128000009)】；

基金：The project was funded by the National Natural Science Foundation of China (Project No. 30500396), and the National High Technology Research and Development Program of China (Project No. 2009AA10Z109).

语种：英文

外文关键词：Apomictic; Apomixis; Embryogenesis; Gametocyte; Seed production

摘要：Female sterile plants are ideal for isolating genes involved in megasporogenesis and for investigating gene functions. Engineered female and male sterilities in plants have been considered as a way to reduce the potential of invasive species by eliminating seed set and to prevent gene flow between genetically modified species. A dwarf mutant 'PM2' induced by carrying 'Nassau' Kentucky bluegrass seeds to outer space lost the ability to produce seeds under either field conditions or artificial flower induction, whereas the untreated control was normal. Comparative study of microspore, macrospore, and embryo sac at different developmental stages along with controlled pollination showed that the PM2 mutant is likely both male and female sterile. The apoporous embryo development in PM2 aborted at globular stage prior to coleoptile formation. Multiple genes may be involved in the mutation based on the evidence observed from the morphology and anatomy of the mutant. (C) 2011 Elsevier B.V. All rights reserved.