文献类型：期刊文献

英文题名：Effects of even- versus uneven-aged tree species (Pinus massoniana and Castanopsis hystrix) mixing on microbial communities across bulk soil, rhizosphere soil, and fine roots

作者：Liu, Runhong[1] Bai, Yuxin[2] He, Peng[1] Cen, Qilan[1] Luo, Xinyu[1] Yang, Liurong[1] Ming, Angang[3,4] Shu, Weiwei[3,4] Shen, Weijun[1]

第一作者：Liu, Runhong

通信作者：Shen, WJ[1]

机构：[1]Guangxi Univ, Coll Forestry, Guangxi Key Lab Forest Ecol & Conservat, Nanning, Peoples R China;[2]Hohhot Branch, Inner Mongolia Environm Monitoring Stn, Hohhot, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Trop Forestry, Pingxiang, Peoples R China;[4]Youyiguan Forest Ecosyst Observat & Res Stn Guangx, Guangxi Youyiguan Forest Ecosyst Res Stn, Pingxiang, Peoples R China

年份：2025

卷号：16

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-105025555871);WOS:【SCI-EXPANDED(收录号:WOS:001640414500001)】；

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This research was financially supported by the Guangxi Science and Technology Base and Talent Special Project (AD23026326, AD25069066), the National Natural Science Foundation of China (32271847, U24A20430), and the Innovation Project of Guangxi Graduate Education (YCBZ2023024, YCSW2024032).

语种：英文

外文关键词：even-aged mixed plantation; uneven-aged mixed plantation; community composition; co-occurrence network; network complexity; keystone taxa

摘要：Introduction Mixed-species plantations are a promising strategy to increase productivity and promote sustainable forest management. However, the effects of even-aged and uneven-aged tree species mixtures on microbial communities along the root-soil continuum remains underexplored.Methods We investigated the bacterial and fungal communities across three distinct microhabitats-bulk soil, rhizosphere soil, and fine roots-within two mixed plantations (even- and uneven-aged) and their respective monocultures.Results Our results show that while even-aged mixing had no significant impact on microbial alpha diversity across the three microhabitats, uneven-aged mixing significantly altered some specific indices, indicating that uneven-aged mixing has a greater impact on microbial alpha diversity than even-aged mixing. Both mixing modes significantly altered microbial community composition, with mixed plantations exhibiting intermediate characteristics between monocultures. Although microbial taxonomic and functional compositions were largely unaffected by even-aged and uneven-aged mixing in most cases, distinct differences emerged across microhabitats. Notably, mixed plantations showed increased complexity in fungal co-occurrence networks and harbored more bacterial and fungal keystone species. Fungal communities were more sensitive to both mixing modes, whereas bacterial communities were more strongly influenced by soil environmental factors, particularly pH, which emerged as the primary driver of microbial variation across all plantation types.Discussion Our findings highlight that the effects of tree species mixing on microbial communities vary significantly with mixing mode, microhabitat, and microbial taxa, and these should be emphasized in future research and silvicultural practices.