文献类型：期刊文献

英文题名：Physiological and transcriptomic regulation of Populus simonii fine roots exposed to a heterogeneous phosphorus environment in soil

作者：Gan, Honghao[1,2] Chu, Jianmin[1] Shi, Wenguang[1,2] Luo, Zhi-Bin[1,2,3,4,5]

第一作者：甘红豪;Gan, Honghao

通信作者：Luo, ZB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm,Key Lab Forest Culti, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China;[3]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry Yellow River Delta, Comprehens Expt Ctr, Dongying 257000, Shandong Provin, Peoples R China;[5]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2024

卷号：219

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001158555400001)】；

语种：英文

外文关键词：Heterogeneous Pi; Phytohormone; Transcription factors; Metabolism; Fine root proliferation

摘要：Soil phosphorus (P) is usually limited and heterogeneously distributed. However, the physiological and transcriptional mechanisms of the woody plant fine roots to heterogeneous P are still unclear. In this study, a split-root system was used to simulate a heterogeneous P environment; that is, half of the fine roots of Populus simonii were grown under normal Pi conditions (2 mM, Hetero-NP), and the other half were grown under low Pi conditions (0.2 mM, Hetero-LP). Subsequently, the physiological and transcriptomic responses of the fine roots were determined under Hetero-NP and Hetero-LP conditions. The concentrations of ATP, malic acid and citric acid in the Hetero-LP region were lower than those in the Hetero-NP region, but the activity of APs was higher. P. simonii fine roots in the Hetero-NP or Hetero-LP region had larger fine root surfaces and SRL, as well as higher malic acid, citric acid, JA and SA concentrations, than those in the homogeneous Pi supply (Homo-NP or Homo-LP). In addition, the soluble protein and total free amino acid concentrations, as well as the AP, PEPC and MDH activities of P. simonii fine roots in Hetero-NP or Hetero-LP were higher than those in Homo-NP but lower than those in Homo-LP. GO, KEGG and MapMan analyses of the differentially expressed genes revealed that the genes in the "metabolic process" term and "plant hormone signal transduction" pathway, as well as in the "RNA", "protein" and "signalling" categories, play key roles in the response of P. simonii fine roots to heterogeneous Pi. Our results indicated that the response of P. simonii fine roots to Hetero-NP or Hetero-LP differ due to local and systematic regulation. Phytohormones and the MAPK signaling system are involved in the local and systematic response of P. simonii fine roots to heterogeneous Pi supplies by promoting fine root elongation and regulating metabolic processes. Taken together, the findings of this study provide new insights into the response of woody plant fine roots to heterogeneous Pi.