文献类型：期刊文献

英文题名：The yellowhorn MYB transcription factor MYB30 is required for wax accumulation and drought tolerance

作者：Liu, Xiaojuan[1] Ban, Zhuo[1] Yang, Yingying[1] Xu, Huihui[1] Cui, Yifan[1] Wang, Chenxue[1] Bi, Quanxin[1] Yu, Haiyan[1] Wang, Libing[1]

第一作者：刘肖娟

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China

年份：2024

卷号：44

期号：10

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85205604448);WOS:【SCI-EXPANDED(收录号:WOS:001326674400001)】；

基金：The authors thank the National Key Laboratory of Forest Genetics and Breeding for providing a good experimental environment.

语种：英文

外文关键词：cuticular wax; drought resistance; transcriptional regulation; Xanthoceras sorbifolium; Xsmyb30

摘要：Yellowhorn (Xanthoceras sorbifolium Bunge) is an economically important tree species in northern China, mainly distributed in arid and semi-arid areas where water resources are scarce. Drought affects its yield and the expansion of its suitable growth area. It was found that the wax content in yellowhorn leaves varied significantly among different germplasms, which had a strong correlation with the drought resistance of yellowhorn. In this study, XsMYB30 was isolated from 'Zhongshi 4' of yellowhorn, a new highly waxy variety. DAP-Seq technology revealed that the pathways associated with fatty acids were significantly enriched in the target genes of XsMYB30. Moreover, the results of electrophoretic mobility shift assay, yeast one hybrid assay and dual-luciferase assay demonstrated that XsMYB30 could directly and specifically bind with the promoters of genes involved in wax biosynthesis (XsFAR4, XsCER1 and XsKCS1), lipid transfer (XsLTPG1 and XsLTP1) and fatty acid synthesis (XsKASIII), thus enhancing their expression. In addition, the overexpression of XsMYB30 in poplar promoted the expression levels of these target genes and increased the wax deposition on poplar leaves leading to a notable improvement in the plant's ability to withstand drought. These findings indicate that XsMYB30 is an important regulatory factor in cuticular wax biosynthesis and the drought resistance of yellowhorn.