文献类型：期刊文献

中文题名：香椿KNOX家族基因鉴定及其在干旱和盐胁迫下的表达分析

英文题名：Identification and Expression Analysis of KNOX Family Genes in Toona sinensis under Drought and Salt Stress

作者：陈舒鑫[1,2] 樊俐娇[1] 贾瑜涵[1] 刘军[1] 华克达[3] 邱勇斌[4] 张能军[4] 卓仁英[1] 韩小娇[1]

第一作者：陈舒鑫

机构：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400;[2]南京林业大学风景园林学院,江苏南京210037;[3]富阳区农业农村局,浙江杭州311400;[4]开化县林场,浙江开化324300

年份：2025

卷号：40

期号：5

起止页码：141-150

中文期刊名：西北林学院学报

外文期刊名：Journal of Northwest Forestry University

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划项目(2021YFD2200305);中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2020SZ004)。

语种：中文

中文关键词：香椿;KNOX基因家族;生物信息学;非生物胁迫

外文关键词：Toona sinensis;KNOX gene family;bioinformatics;abiotic stresses

分类号：S792.99;Q945.78

摘要：KNOX基因家族在植物生长发育及非生物胁迫响应中发挥着关键作用。本研究旨在鉴定香椿KNOX基因家族成员,并分析其在干旱和盐胁迫条件下的表达模式,为探究KNOX基因功能及培育抗逆性更强的香椿新种质提供理论依据。对香椿KNOX基因家族进行全基因组鉴定和分析,其中包括系统发育关系、基因结构、保守基序、染色体定位、顺式作用元件和共线性。通过组织培养获得4周龄生根香椿幼苗后,分别使用15%PEG 6000和100 mmol/L NaCl进行水培处理,并在处理后6、24和96 h分别采集根、茎和叶片样本。此外,另取5年生香椿的叶柄、顶芽、根、茎、叶、木质部和韧皮部,用于RNA提取,利用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)测定TsKNOX基因家族成员的表达水平。在香椿中共鉴定到12个KNOX家族成员,根据其进化关系分析可分为2大亚类。顺式作用元件分析表明,G-Box、Box4和ABRE元件在TsKNOX家族基因的启动子区域高度富集。12个TsKNOX基因分布在11条染色体上。共线性分析表明,10个TsKNOX基因对均是由片段复制产生,证明片段复制是该家族扩张的主要驱动力。qRT-PCR结果表明,KNOXs基因主要在韧皮部和根中高表达;大部分的TsKNOX家族基因在茎中受干旱和盐胁迫显著诱导。该研究通过对香椿KNOX基因家族鉴定和表达模式分析,初步发现TsKNOX基因在香椿响应干旱和盐胁迫中的作用,为进一步解析该家族的基因功能和作用机制奠定了基础。
The KNOX gene family plays an important role in plant growth and development and abiotic stresses.Our aim was to identify the members of the KNOX gene family in Toona sinensis and investigate their response to drought and salt stress.Our study will provide a theoretical foundation for elucidating KNOX gene functions and developing novel stress-resistant T.sinensis germplasm.A genome-wide analysis of the KNOX gene family in T.sinensis was performed,including phylogenetic relationships,gene structure,conserved motifs,chromosomal localization,cis-regulatory elements,and synteny analysis.Four-week-old T.sinensis seedlings from micropropagation,were subjected to hydroponic treatments with 15%PEG 6000 or 100 mmol/L NaCl.Root,stem,and leaf samples were collected at 6,24,and 96 h.Meanwhile,petioles,terminal buds,roots,stems,leaves,xylem and phloem of 5-year-old T.sinensis were also gathered.RNA was further extracted from all the samples.The expression levels of TsKNOX gene family were measured using quantitative real-time PCR(qRT-PCR).A total of 12 KNOX gene family members were identified in T.sinensis,and they can be divided into two subgroups according to their evolutionary relationships.The analysis of cis-regulatory elements revealed a high enrichment of G-Box,Box4,and ABRE elements in the promoter regions of TsKNOX genes.These 12 TsKNOX genes were distributed across 11 chromosomes.Synteny analysis showed that 10 TsKNOX genes resulted from segmental duplication,indicating that segmental duplication is the primary driving force behind the expansion of this gene family.In addition,KNOX genes were predominantly expressed in the phloem and roots,with most TsKNOX genes being significantly induced in the stems under drought and salt stress.This study provides preliminary insights into the role of TsKNOX genes in the response to drought and salt stress,laying the foundation for further research into the specific gene functions and mechanisms within this family.