文献类型：期刊文献

英文题名：Construction of a Core Collection of Germplasms from Chinese Fir Seed Orchards

作者：Wu, Hanbin[1,2] Duan, Aiguo[1,2] Wang, Xihan[3] Chen, Zhiyun[3] Zhang, Xie[4] He, Guiping[5] Zhang, Jianguo[1,2]

第一作者：Wu, Hanbin

通信作者：Zhang, JG[1];Zhang, JG[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing 210037, Peoples R China;[3]State Owned Forestry Farm Weimin, Shaowu 354006, Peoples R China;[4]Hunan Acad Forestry, Inst Forestry, Changsha 410004, Peoples R China;[5]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2023

卷号：14

期号：2

外文期刊名：FORESTS

收录：;EI(收录号：20230913656167);Scopus(收录号：2-s2.0-85149024091);WOS:【SCI-EXPANDED(收录号:WOS:000939122000001)】；

基金：This study was supported financially by Research on Breeding of High-Yield, High-Quality and High-Efficiency New Varieties of Chinese Fir, the National Key Research and Development Project of China of the 14th Five-Year Plan (Grant Number 2022YFD2200201).

语种：英文

外文关键词：Chinese fir; core collection; SSR; seed orchard

摘要：Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is one of the most important tree species for afforestation in China. First-, second-, and third-generation seed orchards of Chinese fir have been established successively, and rich germplasms have been accumulated in the process of genetic improvement. It is necessary to build a core collection of germplasms from Chinese fir seed orchards. In this work, we constructed core collections representing the genetic diversity of Chinese fir seed orchard resources based on SSR data. A total of 607 seed orchard materials from three generations were used to determine the best sampling method and intensity by comparing and analyzing nine methods for constructing core collections. Core Hunter's multi-strategy optimizes allele coverage and the distance criterion under a 30% sampling intensity (weight: A-NE, 0.7; CV, 0.3 and E-NE, 0.5; CV, 0.5), which is superior to other strategies and was selected as the best method. The two core collections (A-NE&CV73, E-NE&CV55) constructed contained all the alleles of the whole collection and effectively limited the homology in the core collections; each core collection contained 182 accessions. Our findings could contribute greatly towards improving the management of genetic resources in Chinese fir seed orchards and provide elite materials for future studies.