文献类型：期刊文献

中文题名：孝顺竹愈伤组织诱导及植株再生

英文题名：Callus Induction and Plantlet Regeneration of Bambusa multiplex

作者：袁金玲[1] 顾小平[1] 李潞滨[2] 岳晋军[1] 姚娜[2] 郭广平[1]

第一作者：袁金玲

机构：[1]中国林业科学研究院亚热带林业研究所,富阳311400;[2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091

年份：2009

卷号：45

期号：3

起止页码：35-39

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(30271099);浙江省自然科学重点基金(ZA0204)资助

语种：中文

中文关键词：孝顺竹;愈伤组织诱导;植株再生;培养基

外文关键词：Bambusa multiplex; callus induction; plant regeneration; media

分类号：S718.46;Q943.1

摘要：利用孝顺竹小穗和种胚为外植体,诱导出胚性愈伤组织并成功实现植株再生。对影响愈伤组织诱导和分化的基本培养基、激素种类及质量浓度等进行筛选,结果表明:愈伤组织诱导以NB,N6基本培养基附加4mg·L-12,4-D较好;外植体在NB+500mg·L-1脯氨酸+500mg·L-1谷氨酰胺+300mg·L-1水解酪蛋白+30g·L-1蔗糖+8g·L-1卡拉胶(Carrageenan)+4mg·L-12,4-D的诱导培养基上经20d培养,小穗愈伤组织诱导率达87.30%,种胚愈伤组织诱导率为76.27%。愈伤组织预分化及分化的基本培养基以MS较适宜,经MS+30g·L-1蔗糖+10g·L-1卡拉胶+4mg·L-1KT培养基预分化7d后,转入无激素的MS培养基上分化14d,小穗愈伤组织仅个别长出绿色芽头;但种胚愈伤组织芽分化率可达80.0%。分化芽中有8%左右的白化苗,并伴有花叶苗出现。优化后的种胚愈伤组织预分化最佳培养基是MS+3mg·L-16-BA+3mg·L-1KT。分化芽苗在添加2mg·L-1NAA的MS培养基上生根良好,移栽成活率可达70%以上。
Embryogenic callus was induced and plant regeneration had been achieved by culturing spikelets and embryo explants of Bambusa multiplex. Basal media, phytohormones and their concentration were screened, and the results showed that NB and N6 basal medium supplemented with 4 mg· L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） were relatively better for callus induction. A 87.30% spikelets and a 76.27% embryos were induced to generate callus on NB medium supplemented with 500 mg· L^-1 proline, 500 mg· L^-1 glutamine, 300 mg· L^-1 hydrolyzed casein, 30 g· L^-1 sucrose, 8 g· L^-1 carrageenan and 4 mg· L^-1 2,4-D in 20 days. Murashige and Skoog （MS） basal medium was effective for embryoids＇ pre-differentiation and germination. After a 7 d pre-differentiation on MS supplemented with 30 g· L^-1 sucrose, 10 g· L^-1 carrageenan and 4 mg· L^-1 kinetin （KT） and a 14 d auxin-free germination culture on MS, only a few spikelet calli germinated; whereas embryo calli regenerated by up to 80.0% . Around 8 % plantlets were albinal, and mosaic plantlets were occasionally found. Optimal pre-differentiation medium for embryo embryoids was MS supplemented with 3 mg· L^-1 6-benzylaminopurine （6-BA） and 3 mg· L^-1 KT. MS supplemented with 2 mg· L^-1 naphthaleneacetic acid （NAA） was effective for the plantlet rooting. Rooted plantlets transferred to soil with over 70% success.