文献类型：期刊文献

英文题名：Preliminary analysis of amplicon high-throughput sequencing as a method for the assessment of fungal diversity in discolored wood

作者：Ma, Xingxia[1] Jiang, Mingliang[1] Liu, Junliang[1] Deng, Hao[1] Wang, Shuangyong[1]

第一作者：马星霞

通信作者：Ma, XX[1]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China

年份：2017

卷号：71

期号：10

起止页码：793-800

外文期刊名：HOLZFORSCHUNG

收录：;EI(收录号：20174104255850);Scopus(收录号：2-s2.0-85030687971);WOS:【SCI-EXPANDED(收录号:WOS:000412101400004)】；

基金：This research was supported financially by the Central-level Public Welfare Foundation of the Research Institute of Forest New Technology, Chinese Academy of Forestry (CAFYBB2016SY024) and the China National Natural Science Foundation, Ministry of Human Resources and Social Security of the People's Republic of China (2016).

语种：英文

外文关键词：amplicon high-throughput sequencing; discolored wood; fungal community structure; fungal diversity; stain fungi

摘要：The diversity of stain fungi is important if wood is inhabited with various fungi, and the discoloration mechanism will be better understood. MiSeq amplicon high-throughput sequencing (Illumina (R)) is able to detect species richness (the number of species within a community) and species evenness (the sizes of species populations within a community). This study detected fungal diversity in discolored Mongolian pine for the first time by the MiSeq approach, focusing on the nuclear ribosomal internal transcribed spacer-1 (ITS1). The results show that the discolored wood was inhabited by a combination of microorganisms, more than 90% of which belong to Ascomycota fungi at the phylum level. The MiSeq method revealed not only all the inhabited fungal species but also their quantitative relation to each other. The dominant fungal species in sample A are Helotiales (34.1%) and Hypocreales (20.7%). The dominant fungal species in sample B is Nectriaceae (67.9%), while Hypocrea (34.7%) and Sporothrix (27.6%) are the dominant fungal species in sample C. It was confirmed via core microbiome analysis that the following fungi were common stain fungi in the three discolored samples: Fusarium spp., Aspergillus spp., Sporothrix spp., Penicillium spp., Trichoderma spp., Alternaria spp. and Cladophialophora spp.