文献类型：期刊文献

中文题名：峨眉山地区杨叶木姜子群体遗传多样性研究

英文题名：A Study on Population Genetic Diversity among Litsea populifolia(Hemsl.)Gamble in Mount Emei Area

作者：王雪[1] 高暝[1] 吴立文[1] 汪阳东[1] 刘红彩[2] 陈益存[1]

第一作者：王雪

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]陕西佛坪国家级自然保护区管理局,汉中723400

年份：2019

卷号：0

期号：2

起止页码：359-369

中文期刊名：植物遗传资源学报

外文期刊名：Journal of Plant Genetic Resources

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：浙江省农业(林木)新品种选育重大科技专项(2016C02056)~~

语种：中文

中文关键词：杨叶木姜子;峨眉山;GBS;遗传结构;遗传多样性

外文关键词：Litsea populifolia(Hemsl.)Gamble;Emei Mountain;GBS;genetic structure;genetic diversity

分类号：S792.99

摘要：杨叶木姜子在中医药、天然香精香料、趋避剂等领域具有重要应用价值,但其分布地域狭窄,仅在四川省部分地区分布,严重影响杨叶木姜子的开发和利用。本研究以四川盆地西南边缘峨眉山地带的杨叶木姜子为材料,在峨眉山全分布区内根据地理位置和海拔高度的差异选择7个不同区域采集到84个样本,利用简化基因组测序(GBS)技术对其进行遗传多样性分析。基于GBS文库,共检测获得到48795个SNP位点,主要变异来源于内含子区域(47.69%)和基因间区(30.71%)。系统进化树分析、主成分分析和群体结构分析均显示7个杨叶木姜子居群聚类到3个大群体。这3个群体分别来自不同的祖先,分别位于峨眉山以金顶为中心向外延伸的2条主要山脉和峨眉山断层向外延伸的山谷区域中,表明峨眉山区地理差异是造成遗传多样性的主要因素。3个群体平均观测杂合度(Ho)为0.27218,平均期望杂合度(He)为0.24596。群体内变异百分率达到96.28%,而群体间变异仅为3.72%。结果表明,峨眉山杨叶木姜子群体间具有较低的遗传多样性水平,但其在群体内具有较高的核苷酸多样性,变异主要来源于群体内变异。研究结果为杨叶木姜子遗传育种和物种多样性保护提供依据,在家系选择方面需注意群体内大量采样,种质资源保存应尽量选择较低海拔。
Litsea populifolia(Hemsl.)Gamble,a deciduous arbor of Lauraceae,has potential and extensive application in Traditional Chinese Medicine(TCM),cosmetics and edible essence.However,this species distributes in a narrow geographical region(restricted in Sichuan province,China),which seriously prohibits the exploitation and utilization of L.populifolia.(Hemsl.)Gamble.Understanding the genetic diversity of populations among L.populifolia(Hemsl.)Gamble is urgently necessary and helpful for the future breeding and diversity conservation.In this study,the genetic diversity of 84 leaf samples,which were collected from 7 different regions in Mount emei area,was analyzed by genotyping-by-sequencing(GBS)technique.After the construction of GBS library using standard pipeline,we identified 48795 SNP sites globally,47.69% and 30.71% of which were located in intronic regions and intergenic regions,respectively.According to the phylogenetic tree,principal components and population structure analysis,seven subgroups of samples were clustered into three clusters.The average of Ho and He was 0.27218 and 0.24596,respectively.As a result of ANOVA,the percentage of intragroup variation reached 96.28%,while inter-group variation was only 3.72%.These results indicated that regional divergence issued the main factor of genetic diversity among L.populifolia(Hemsl.)Gamble in Emei Mountain,and the different altitudes in high altitude area also affected the genetic diversity of intra-population.The high nucleotide diversity in the populations and the high percentage of intra-group variation illustrated that genetic variation of L.populifolia(Hemsl.)Gamble in Emei Mountain mainly exists in intra-population.The characteristics of dioecism and cross-pollination in L.populifolia(Hemsl.)Gamble caused higher gene flow.However,due to the fragmentation of its habitat and the complex vertical climate,there was a low gene ?ow between the groups,causing obvious population differentiation and the variation mainly within the populations.Our data provided a fundamental basis for the genetic breeding and diversity conservation of L.populifolia(Hemsl.)Gamble.In terms of family line selection,a large number of samples should be included in the population,and the preservation of germplasm resources should be at a lower altitude as far as possible.