文献类型：期刊文献

中文题名：MicroRNA319-mediated gene regulatory network impacts leaf development and morphogenesis in poplar

作者：Yanxia Cheng[1,2] Lihu Wang[3] Manzar Abbas[4] Xiong Huang[2,5] Qiao Wang[2,5] Aimin Wu[6,7] Hairong Wei[8] Shaobing Peng[1] Xinren Dai[2] Quanzi Li[2,5]

第一作者：Yanxia Cheng

机构：[1]College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,P.R.China;[2]State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,P.R.China;[3]College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan,Hebei 056038,P.R.China;[4]Key Laboratory of Aromatic Plant Resources Exploitation and Utilization,School of Agriculture,Forestry and Food Engineering,Yibin University,Yibin,Sichuan 644000,P.R.China;[5]Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,P.R.China;[6]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou,Guangdong 510642,P.R.China;[7]Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642,P.R.China;[8]College of Forest Resources and Environmental Science,Michigan Technological University,Houghton,Michigan 49931,USA These authors contributed equally:Yanxia Cheng,Lihu Wang,Manzar Abbas

年份：2021

卷号：1

期号：1

起止页码：20-29

中文期刊名：林学研究(英文)

外文期刊名：Forestry Research

基金：supported by a grant from Fundamental Research Funds of CAF(CAFYBB2017ZY001).

语种：英文

中文关键词：network;bundle;TCP;

分类号：TP3

摘要：MicroRNA319(miR319)has been implicated in leaf development in a number of plant species.Here we study the roles of miR319a and its regulated network in leaf development in poplars.Over-expression of miR319a in Populus alba×Populus glandulosa caused dwarf statures,narrow leaf blades and serrated leaf margins.The vascular bundles and bundle sheaths in transgenic leaves had more layers of cells than those in the leaves of control plants,indicating enhanced lignification in these cells.Among the 93 putative targets of miR319a predicted with the psRNATarget tool,only three genes,TCP(TEOSINTE BRANCHED1,CYCLOIDEA,and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR),were differentially expressed in the leaves of MIR319a-over-expression transgenic lines.With the RNA-seq data sets from multiple MIR319a over-expression transgenic lines,we built a three-layered gene regulatory network mediated by miR319a using Top-down graphic Gaussian model(GGM)algorithm that is capable of capturing causal relationships from transcriptomic data.The results support that miR319a primarily regulates the lignin biosynthesis,leaf development and differentiation as well as photosynthesis via miR319-MEE35/TCP4,miR319-TCP2 and miR319-TCP2-1 regulatory modules.