文献类型：期刊文献

中文题名：氮素形态及配比对雷竹光合特性和氮代谢酶活性的影响

英文题名：Effects of nitrogen forms and ratios on photosynthetic characters and enzyme activities in nitrogen metabolism of Phyllostachys violascens

作者：叶莉莎[1] 陈双林[1]

第一作者：叶莉莎

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2016

卷号：35

期号：9

起止页码：2355-2360

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：浙江省中国林业科学研究院省院合作项目(2013SY04);中央级公益性科研院所基本科研业务费专项资金项目(RISF61258)资助

语种：中文

中文关键词：硝态氮;铵态氮;光合参数;硝酸还原酶;谷氨酰胺合成酶

外文关键词：nitrate nitrogen ; ammonium nitrogen ; photosynthetic character; nitrate reductase ;glutamine synthetase

分类号：S572

摘要：为给雷竹林的合理施用氮肥提供科学依据,通过盆栽试验,设置了硝铵比(NO_3^--N∶NH_4^+-N)为1∶0、2∶1、1∶1、1∶2、0∶1的5个氮素形态营养处理,测定了雷竹叶片叶绿素a(Chla)、叶绿素b(Chlb)、类胡萝卜素(Car)含量和净光合速率(Pn)、气孔导度(Gs)、胞间CO_2浓度(Ci)、蒸腾速率(Tr)光合参数以及硝酸还原酶(NR)、谷氨酰胺合成酶(GS)活性。结果表明:随氮素供应中NH_4^+-N比例的增加,雷竹叶片Chla、Chlb、Car含量和Pn、Gs、Tr均呈先升高后降低的变化趋势,硝铵比1∶2处理明显高于其他处理,而Ci出现相反的变化规律,在硝铵比1∶2处理时明显低于其他处理;NR、GS活性随NH_4^+-N比例的增加也呈先升高后降低的变化趋势,且NH_4^+-N/NO_3^--N混合营养的处理间无显著差异,均高于单一氮素形态的营养处理。综合分析认为,雷竹为弱喜铵植物,但硝铵混合营养更能促进雷竹光合作用和氮同化能力。
In order to provide scientific guidance for nitrogen fertilization in Phyllostachys violascens stands, a pot experiment was carried out with five ratios of NO3^--N∶NH4^＋-N at 1∶0, 2∶1, 1∶1, 1∶2 and 0∶1. Measured indicators included chlorophyll a （Chla）, chlorophyll b （Chlb） and carotenoids （Car） contents, net photosynthetic rate （Pn）, stomatal conductance （Gs）, intercellular CO2 concentration （Ci）, transpiration rate （Tr）, and activities of nitrate reductase （NR） and glutamine synthetase （GS） in leaves of Phyllostachys violascens. Results showed that, with increasing NH4^＋-N proportion in nitrogen supply, the photosynthetic pigment contents, Gs, Ci and Tr rose at first and then fell. These variables under the treatment of ammonium/nitrate ratio at 1∶2 were significantly higher than those under the other treatments, while Ci variation was opposite. With increasing the proportion of NH4^＋-N, the activities of NR and GS showed a trend of increase at first and then decrease, being not significantly different among the mixed nutrition treatment, but higher in the mixed nutrition treatments than in the single nitrogen treatments. Overall, it might be concluded that P. violascensis a kind of plant that slightly prefers ammonium, and that mixture of ammonium and nitrate can facilitate leaf photosynthesis and nitrogen assimilation.