文献类型：期刊文献

英文题名：Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

作者：Wu, Qikui[1,2,3] Chen, Chen[1] Wang, Xiaojun[1] Zhang, Zihan[1,4,5] Yu, Fangyuan[1] Guy, Robert D.[2]

第一作者：Wu, Qikui

通信作者：Yu, FY[1];Guy, RD[2]

机构：[1]Nanjing Forestry Univ, Coll Forest Sci, Collaborat Innovat Ctr Sustainable Forestry South, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China;[2]Univ British Columbia, Fac Forestry, Dept Forest & Conservat Sci, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada;[3]Shandong Agr Univ, State Forestry & Grassland Adm, Key Lab Silviculture Downstream Areas Yellow Rive, Tai An, Shandong, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, State Forestry Adm, Res Inst Forestry, Beijing, Peoples R China;[5]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Res Inst Forestry, Beijing, Peoples R China

年份：2021

卷号：101

期号：14

起止页码：6053-6063

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000644484700001)】；

基金：We thank Mr Jihua Wang (Jiangsu Guoxing Co. Ltd) for providing Styrax tonkinensis seeds. This work was supported by the Doctorate Fellowship Foundation of Nanjing Forestry University, Joint Research Project Based on Cooperative Program for Bachelor of Science in Forestry by Nanjing Forestry University and University of British Columbia, National Natural Science Foundation of China (3197140894) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：lipid accumulation; seed development; differentially expressed proteins; clustering analysis; regulatory network

摘要：BACKGROUND Styrax tonkinensis is a white-flowered tree with considerable potential as a feedstock source for biodiesel production from the oily seed contained within its nutlike drupes. Transcriptome changes during oil accumulation have been previously reported, but not concurrent changes in the proteome. RESULTS Using proteomic analysis of samples collected at 50, 70, 100 and 130 days after flowering (DAF), we identified 1472 differentially expressed proteins (DEPs). Based on their expression patterns, we grouped the DEPs into nine clusters and analyzed the pathway enrichment. Proteins related to starch and sucrose metabolism were most abundant at 50 DAF. Proteins involved in fatty acid (FA) biosynthesis were mainly grouped into a cluster that peaked at 70 DAF. Proteins related to protein processing in endoplasmic reticulum had two major patterns, trending either upwards or downwards, while proteins involved in amino acid biosynthesis showed more complex relationships. We identified 42 key enzymes involved in lipid accumulation during kernel development, including the acetyl-CoA carboxylase complex (ACC) and the pyruvate dehydrogenase complex (PDC). One oil body membrane protein, oleosin, continuously increased during kernel development. CONCLUSION A regulatory network of oil accumulation processes was built based on protein and available transcriptome expression data, which were in good temporal agreement. This analysis placed ACC and PDC in the center of the network, suggesting that the glycolytic provision of substrate plays a central regulatory role in FA biosynthesis and oil accumulation.