文献类型：期刊文献

英文题名：Comparative Metabolomic Analysis of the Cambium Tissue of Non-transgenic and Multi-Gene Transgenic Poplar (Populus x euramericana 'Guariento')

作者：Ning, Kun[1,2] Ding, Changjun[1,2] Zhu, Wenxu[3] Zhang, Weixi[1,2] Dong, Yufeng[4] Shen, Yingbai[5] Su, Xiaohua[1,2,6]

第一作者：Ning, Kun

通信作者：Su, XH[1];Su, XH[2];Su, XH[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing, Peoples R China;[2]State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[3]Shenyang Agr Univ, Coll Forestry, Shenyang, Liaoning, Peoples R China;[4]Shandong Acad Forestry, Shandong Prov Key Lab Forest Tree Genet Improveme, Jinan, Shandong, Peoples R China;[5]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing, Peoples R China;[6]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern Chin, Nanjing, Jiangsu, Peoples R China

年份：2018

卷号：9

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000441884200001)】；

基金：This study was supported by the Basic Research Fund of RIF (Grant No. CAFYBB2017SY001), the National High-Tech R&D Program of China (863 Program) (Grant No. 2011AA100201), and the National Key Program on Transgenic Research (Grant No. 2018ZX08021001).

语种：英文

外文关键词：metabolomics; multi-gene transgenic poplar; cambium; GC-MS; UPLC-MS/MS

摘要：Poplar, a model for woody plant research, is the most widely distributed tree species in the world. Metabolites are the basis of phenotypes, allowing an intuitive and effective understanding of biological processes and their mechanisms. However, metabolites in non-transgenic and multi-gene transgenic poplar remains poorly characterized, especially in regards of the influences on quantity and in the analysis of the relative abundance of metabolites after the introduction of multi stress-related genes. In this study, we investigated the cambium metabolomes of one non-transgenic (D5-0) and two multi-gene (vgb, SacB, JERF36, BtCry3A, and OC-I) transgenic lines (D5-20 and D5-21) of hybrid poplar (Populus x euramericana 'Guariento') using both gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We aimed to explore the effects of the exogenous genes on metabolite composition and to screen out metabolites with important biological functions. Finally, we identified 239 named metabolites and determined their relative abundance. Among these, 197 metabolites had a different abundance across the three lines. These methabolites spanned nine primary and 44 secondary metabolism pathways. Arginine and glutamate, as substrates and intermediates in nitrogen metabolism, and important in growth and stress-related processes, as well as sucrose, uridine diphosphate glucose, and their derivatives, precursors in cell wall pathways, and catechol, relevant to insect resistance, differed greatly between the genetically modified and non-transgenic poplar. These findings may provide a basis for further study of cambium metabolism, and fully understand metabolites associated with stress response.