文献类型：期刊文献

英文题名：Establishment of an efficient genetic transformation system: A case study of RNAi-induced silencing of the transcription factor MeGI in Diospyros oleifera Cheng seedlings

作者：Mai, Yini[1] Liu, Yang[2] Yuan, Jiaying[1] Ye, Lingshuai[1] Zhang, Yue[1] Diao, Songfeng[1] Han, Weijuan[1] Suo, Yujing[1] Li, Huawei[1] Hu, Ruiyang[3] Sun, Peng[1] Li, Ze[2] Fu, Jianmin[1]

第一作者：Mai, Yini

通信作者：Sun, P[1];Fu, JM[1];Li, Z[2]

机构：[1]Chinese Acad Forestry, Res Inst Nontimber Forestry, Key Lab Nontimber Forest Germplasm Enhancement & U, Zhengzhou 450003, Peoples R China;[2]Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China

年份：2023

卷号：308

外文期刊名：SCIENTIA HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-85138786791);WOS:【SCI-EXPANDED(收录号:WOS:000879906700003)】；

基金：This study was supported by the National Natural Science Founda- tion of China (32071801) and the National Key R & D Program of China (2018YFD1000606 and 2019YFD1000600) .

语种：英文

外文关键词：D; oleifera; MeGI; RNAi; Genetic transformation; Plant tissue culture

摘要：Persimmon (Diospyros) is an important genus of temperate fruit trees that originated in East Asia. High-efficiency genetic transformation in Diospyros spp. remains challenging, limiting studies of gene function and the use of modern technology, such as gene editing, in breeding programs. In this study, we established an efficient genetic transformation method based on the infection of hypocotyls and radicles obtained from immature D. oleifera seeds. In particular, we focused on optimising methods for infection and induction of calluses and adventitious buds. RNA interference vectors targeting the transcription factor MeGI, which has been reported to have a feminising effect, were transformed into the hypocotyls and radicles of D. oleifera, producing five positively transformed seedlings. Compared with the wild-type controls, the positively transformed seedlings exhibited dwarfed adventitious buds and narrower leaves. The phenotypic characteristics of the flowers of these positively transformed seedlings will be observed at a later date; this will not only confirm the function of MeGI in Diospyros spp. but also potentially induce the development of male floral buds in gynoecious species and further promote the development of breeding methods. The proposed genetic transformation protocol will be valuable for in-vestigations into gene function and the development of persimmon breeding programs.