文献类型：期刊文献

英文题名：Multi-omics sequencing provides insight into floral transition inCatalpa bungei. CA Mey

作者：Wang, Zhi[1] Ma, Wenjun[1] Zhu, Tianqing[1] Lu, Nan[1] Ouyang, Fangqun[1] Wang, Nan[1] Yang, Guijuan[1] Kong, Lisheng[2] Qu, Guanzheng[3] Zhang, Shougong[1] Wang, Junhui[1]

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Peoples R China

年份：2020

卷号：21

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000555460600006)】；

基金：This work was supported by Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002) and Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZA001-8). The funders had no role in the study design, data analysis and interpretation, and manuscript writing, and only provided financial support.

语种：英文

外文关键词：Floral transition; RNA sequencing; WGCNA; Early flowering; Catalpa bungei

摘要：Background Floral transition plays an important role in development, and proper time is necessary to improve the value of valuable ornamental trees. The molecular mechanisms of floral transition remain unknown in perennial woody plants. "Bairihua" is a type ofC. bungeithat can undergo floral transition in the first planting year. Results Here, we combined short-read next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing to provide a more complete view of transcriptome regulation during floral transition inC. bungei. The circadian rhythm-plant pathway may be the critical pathway during floral transition in early flowering (EF)C. bungei, according to horizontal and vertical analysis in EF and normal flowering (NF)C. bungei. SBP and MIKC-MADS-box were seemingly involved in EF during floral transition. A total of 61 hub genes were associated with floral transition in the MEturquoise model with Weighted Gene Co-expression Network Analysis (WGCNA). The results reveal that ten hub genes had a close connection with theGASA homologue gene(Cbu.gene.18280), and the ten co-expressed genes belong to five flowering-related pathways. Furthermore, our study provides new insights into the complexity and regulation of alternative splicing (AS). The ratio or number of isoforms of some floral transition-related genes is different in different periods or in different sub-genomes. Conclusions Our results will be a useful reference for the study of floral transition in other perennial woody plants. Further molecular investigations are needed to verify our sequencing data.