文献类型：期刊文献

英文题名：Pinirhizobacter soli gen. nov., sp. nov., a novel low temperature resistant gammaproteobacterium in the family Rhodanobacteraceae isolated from rhizospheric soil of Larix gmelinii

作者：Xue, Han[1] Piao, Chun-gen[1] Lin, Ying-hua[2] Li, Yong[1]

通信作者：Li, Y[1]

机构：[1]Chinese Acad Forestry, Key Lab Forest Protect Natl Forestry & Grassland, Ecol & Nat Conservat Inst, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Inst Wetland Res, Beijing 100091, Peoples R China

年份：2022

卷号：204

期号：5

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85147942355);WOS:【SCI-EXPANDED(收录号:WOS:000787764600001)】；

基金：This work was funded by the National Infrastructure of Microbial Resources (NIMR-2020-7) and the National Natural Science Foundation of China (Grant NO. 31570603).

语种：英文

外文关键词：Pinirhizobacter soli gen; nov; Sp; nov; Strain NC2-4-308(T); Taxonomy

摘要：Three yellow-colored strains, NC2-4-308(T), NC3-4-326 and NA3-4-109, were isolated from the rhizosphere soil of Larix gmelinii in Nanwenghe Nature Reserve, Great Khingan, China. These strains were oxidase- and catalase-positive and Gram-staining-negative. The cells were non-motile short rods that were aerobic and non-spore-forming. Growth occurred at pH values of 5.0-8.0 and at 0-4% (w/v) NaCl. The three strains were resistant to low temperature and grew at 2-35 degrees C. The principal fatty acids (> 5%) were summed feature 9, iso-C-15:0, iso-C-17:0 and anteiso-C-15:0. The predominant quinone was ubiquinone-8. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids, three unidentified lipids and three unidentified aminophospholipids. The DNA G + C content of the type species was 64.0 mol%. The 16S rRNA gene sequence similarities among the three strains are more than 99.9%, indicating they belong to the same species. Phylogenetic analysis of the 16S rRNA gene, whole-genome sequences, the low ANI (74.2-75.5%) and dDDH (19.3-20.1%) hybridization values enabled differentiation of strains NC2-4-308(T), NC3-4-326 and NA3-4-109 from the members of related genera. The combined data from the morphological, physiological, biochemical and chemotaxonomic tests indicate the three strains as a novel genus and a novel species in the family Rhodanobacteraceae. Therefore, we propose a novel genus with the name Pinirhizobacter soli gen. nov., sp. nov., for which the type strain is NC2-4-308(T) (= CFCC 14693(T) = KCTC 72394(T)).