文献类型：期刊文献

英文题名：Characterization of Rosa chinensis CBF Genes and the Function of RcCBF6 in Cold Tolerance

作者：Li, Jianbo[1,2] Zheng, Guangshun[1] Li, Shaofeng[1] Tang, Zhimin[1] Sun, Jingshuang[1] Xin, Xuebing[1]

第一作者：李建波

通信作者：Li, JB[1];Sun, JS[1];Xin, XB[1];Li, JB[2]|[a000547af0b9516fa7bc5]辛学兵;

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：63

期号：4

起止页码：267-278

外文期刊名：JOURNAL OF PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85083732349);WOS:【SCI-EXPANDED(收录号:WOS:000528097400002)】；

基金：This work was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2016MA008, CAFYBB2017ZD005 and CAFYBB2019ZY003).

语种：英文

外文关键词：CBF; Phylogenetic analysis; Transgene; Cold tolerance; Rosa chinensis

摘要：As a class of AP2 family transcription factors, C-repeat binding factor (CBF) gene family plays an important role in resisting low-temperature stress and improving cold tolerance of plants. Rosa chinensis is an important horticultural and ornamental plant, but little is known about the molecular characteristics of CBF genes in R. chinensis. In our study, six RcCBFs members were identified at R. chinensis whole genome, including one member RcCBF1 on Chromosome 1 and other five members RcCBF2-6 on Chromosome 7. The analysis of cis-acting elements indicated that there were various cis-acting elements related to stress, development, hormone, and light. The expression profiles showed that most of RcCBFs were mainly expressed in root, and the expression levels of RcCBFs were significantly induced by low-temperature stress, especially RcCBF6. To verify the function of RcCBF6, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The RcCBF6-overexpressing plants exhibited higher tolerance to cold stress as evidenced by a better growth and higher antioxidative enzyme activities than the wild-type plants. Furthermore, the expression levels of some cold-response genes were up-regulated in the transgenic plants, such as KIN1, RD29A, LTP3, and GOLS3. Our study contributes to a better understanding of RcCBF gene family and provides a foundation for the further functional research of RcCBFs.