文献类型：期刊文献

英文题名：The impact of anthelmintic treatment on gut bacterial and fungal communities in diagnosed parasite-free sika deer Cervus nippon

作者：Hu, Xiaolong[1] Xu, Yongtao[2] Liu, Gang[3] Hu, Defu[4] Wang, Yihua[5] Zhang, Weiwei[2] Zheng, Yunlin[1]

第一作者：Hu, Xiaolong

通信作者：Zheng, YL[1];Zhang, WW[2]

机构：[1]Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang, Jiangxi, Peoples R China;[2]Jiangxi Agr Univ, Coll Forestry, Nanchang, Jiangxi, Peoples R China;[3]Chinese Acad Forestry, Inst Wetland Res, Beijing, Peoples R China;[4]Beijing Forestry Univ, Sch Ecol & Nat Conservat, Beijing, Peoples R China;[5]Chinese Acad Forestry, Inst Forest Ecol Environm & Protect, Beijing, Peoples R China

年份：2020

卷号：104

期号：21

起止页码：9239-9250

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000569553400001)】；

基金：This work was supported by the Department of Education of Jiangxi Province (GJJ180228).

语种：英文

外文关键词：Deer; Anthelmintic treatment; Bacteria; Fungi; Parasite

摘要：The gut microbiota, including both bacterial and fungal communities, plays vital roles in the gut homeostasis of animals, and antibiotics can lead to disorders of these microbial communities. The use of anthelmintic treatment to control parasitic infection has long been a standard practice, although its impact on the gut microbiota of healthy sika deer is relatively unknown. This study used next-generation sequencing based on 16S/18S/ITS rRNA genes to investigate the shifts in fecal bacterial and fungal communities in parasite-free sika deer after treatment with fenbendazole and ivermectin tablets. The alpha-diversity of both bacterial and fungal communities was significantly decreased (P< 0.05) after treatment, as were the bacterial genusBacteroidesand fungal genusCandida(P< 0.05). The results of beta-diversity, LEfSe analysis, core community's analysis, taxonomic composition, and functional prediction of fungal and bacterial communities confirmed the substantial impacts of anthelmintic treatment on the function and structure of the intestinal microbiota of sika deer. Nevertheless, many lines of evidence, including beta-diversity, LEfSe analysis and functional prediction analysis, suggested that the anthelmintics exerted more significant influences on fungal communities than on bacterial communities, suggesting that more attention should be paid to the changes in fungal communities of sika deer under anthelmintic treatment. The present study provides evidence to support the assumption that anthelmintic drugs modify the gut microbiota of deer and serves as the first trial to test the potential effects of anthelmintics on mycobiota in ruminants using high-throughput sequencing techniques.