文献类型：期刊文献

中文题名：花绒寄甲辨别光肩星天牛蛀食不同树木所产生虫粪的挥发性化学信号

英文题名：Volatile chemical cue for Dastarcus helophoroides( Coleoptera: Bothrideridae) discriminating the larval frass of Anoplophora glabripennis( Coleoptera: Cerambycidae) larvae feeding on different host tree species

作者：魏建荣[1] 苏智[2] 董丽君[1]

第一作者：魏建荣

机构：[1]河北大学生命科学学院;[2]中国林业科学研究院沙漠林业实验中心

年份：2015

卷号：34

期号：10

起止页码：2814-2820

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金项目(30771738和31070583)资助

语种：中文

中文关键词：花绒寄甲;光肩星天牛;α-古巴烯;蛀干害虫;三级营养;益它素

外文关键词：Dastarcus helophoroides ; Anoplophora glabripennis ; （-） -α-copaene ; wood borer;tritrophic interactions ; kairomone.

分类号：Q969.94;S763.30

摘要：光肩星天牛(Anoplophora glabripennis)危害多种阔叶树种,曾对"三北"防护林造成重大灾害。花绒寄甲(Dastarcus helophoroides)对光肩星天牛取食不同寄主树如杨树、柳树、榆树和槭树后排出的虫粪表现出不同的趋向性,但对槭树的虫粪未表现出趋向性。为了阐明花绒寄甲成虫差异性趋向选择机制,我们采用动态顶空吸附技术收集不同寄主树的虫粪挥发物,利用气相色谱、固相微萃取-气相色谱-质联仪对虫粪中单萜、倍半萜进行定性定量分析。结果表明,萜烯类是虫粪挥发物的主要成分,且源自不同寄主树木虫粪的萜烯释放量相差显著。检测到的所有化合物中,只有α-古巴烯在源于复叶槭的光肩星天牛虫粪中含量极微,而在源于其他5种寄主树的虫粪中含量均较大。在进一步的Y型嗅觉仪生测实验中,α-古巴烯能够显著地吸引花绒寄甲。α-古巴烯可能是花绒寄甲辨别取食不同寄主树的光肩星天牛虫粪的重要信号化合物。
Anoplophora glabripennis is a destructive wood-borer attacking many deciduous hard- wood tree species, especially in northern China. Dastarcus helophoroides parasitizes larvae and pupae of A. glabripennis. Olfactory response of one population of D. helophoroides to larval frass of A. glabripennis from six different host tree species in the genera Populus, Salix, Ulmus and Acer were different, and the larval frass from Acer negundo was not attractive to the population of D. helophoroides. In order to explore the reason that different larval frass of A. glabripennis from dif- ferent host tree species have different attractive capacities to D. helophoroides, frass volatiles were extracted by solid-phase microextraction （SPME） and analyzed by gas chromatography-mass spectrometry （GC-MS）. The monoterpenes released from frass were extracted by Porapak Q and quantified by gas chromatography. Results showed that terpenes were the major components, and their release amounts differed significantly among the tree species. Among all compounds, （-）-α- copaene was the only compound that was at a trace level in the larval frass from A. negundo but detectable in the larval frass from the other five tree species. Further Y-tube bioassays indicated that （-）-α-copaene could attract D. helophoroides adults at a low release rate. Therefore, （-）-α- copaene might serve as the kairomone for D. helophoroides discriminating the larval frass of their hosts feeding on different host tree species.