文献类型：期刊文献

中文题名：不同预处理方法对玉米秸秆酶解性能的影响

英文题名：Effect of different pretreatments on the enzymolysis properties of corn stalk

作者：田庆文[1,2] 房桂干[1,2,3] 沈葵忠[1,2,3] 刘雯雯[1,2] 梁龙[1,2]

第一作者：田庆文

机构：[1]中国林业科学研究院林产化学工业研究所,国家林业局林产化学工程重点开放性实验室,南京210042;[2]南京林业大学林业资源高效加工利用协同创新中心,南京210037;[3]中国林业科学研究院林业新技术研究所,北京100091

年份：2017

卷号：2

期号：2

起止页码：89-94

中文期刊名：林业工程学报

外文期刊名：Journal of Forestry Engineering

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：“十二五”国家科技支撑计划课题(2014BAD02B02)

语种：中文

中文关键词：预处理;玉米秸秆;酶解;还原糖得率;聚糖转化率

外文关键词：pretreatment; corn stalk; enzymolysis; reducing sugars yield; polysaccharide conversion

分类号：TQ401

摘要：研究了NaOH、Ca(OH)_2、NH_3·H_2O和NaHSO_3等4种化学联合盘磨预处理方法对玉米秸秆酶解性能的影响,考察了不同预处理方法对物料得率、木质素脱除率、还原糖得率、聚糖转化率和结晶度的影响。结果表明,预处理后,玉米秸秆的结晶度均降低。机械盘磨可以减小纤维长度和颗粒尺寸,增大比表面积,暴露出更多的纤维素活性位点,增加纤维素和纤维素酶的反应活性,提高其酶解性能。确定了酶解适宜的条件:纤维素酶用量30 U/g,β-葡萄糖苷酶用量10 U/g,酶解温度50℃和时间72 h,在此条件下NaOH、Ca(OH)_2、NH_3·H_2O和NaHSO_3联合盘磨预处理后玉米秸秆的还原糖得率分别为41.00%,23.02%,65.77%和22.22%,聚糖转化率分别为39.04%,18.53%,70.49%和21.33%。在最优条件下,NH_3·H_2O联合盘磨预处理玉米秸秆的还原糖得率和聚糖转化率最高,是一种具有前景的预处理方法。
The effects of different chemical pretreatment methods with NaOH, Ca（OH） 2, NH3·H2O and NaHSO3 associated with mechanical refining on the enzymatic hydrolysis of corn stalk were evaluated. The solid yield, delignification rate, reducing sugars yield, polysaccharide conversion and crystallinity of the corn stalk resulted from the four aforementioned pretreatment methods were compared. The crystallinity of the corn stalk decreased after the four pretreatments, indicating that the crystal structure of the raw materials was damaged to a certain extent during the chemi- cal pretreatment combined with the grinding process. The length of the fibers and size of the corn stalk particles all decreased while the specific surface area increased after the mechanical disk grinding. More active sites of the cellulose were exposed after the pretreatment, resulting in more adequate contact between cellulose and cellulase, and therefore enhancing the efficiency of the enzymatic process. The optimum enzymatic performance of cellulose was obtained at 50℃ for 72 h with the cellulase and glucosidase doses of 30 U/g and 10 U/g, respectively. Under the optimal condition, the reducing sugars yields of corn stalk from pretreatment by NaOH, Ca（OH） 2, NH3 ·H2O and NaHSO3 associated with mechanical refining were 41.00%, 23.02%, 65.77% and 22. 22%, and the polysaccharide conversions were 39. 04%, 18. 53% ,70. 49% and 21.33%, respectively. Furthermore, the reducing sugars yield of corn stalk also increased after mechanical refining. Corn stalk pretreated by NH3·H2O with mechanical refiner gave the highest reducing sugar yield and polysaccharide conversion.