文献类型：期刊文献

中文题名：兰花枯萎病拮抗细菌的筛选与鉴定

英文题名：Screening and Identification of Antagonistic Bacterium against Colletoichum wilt

作者：连彩[1,2] 郭晓军[2] 朱宝成[2] 王建明[1] 张超[1] 曹晓璐[3] 姚娜[3]

第一作者：连彩

机构：[1]山西农业大学农学院;[2]河北农业大学生命科学学院;[3]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室

年份：2012

卷号：27

期号：2

起止页码：222-225

中文期刊名：华北农学报

外文期刊名：Acta Agriculturae Boreali-Sinica

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：海南省科技厅重大科技研发专项(080102)

语种：中文

中文关键词：尖孢镰刀菌;拮抗细菌;筛选;鉴定;Bacillus;velezensis

外文关键词：Fusarium oxysporium; Antagonistic bacteria; Screening; IdentificationIdentification; Bacillus China velezensis

分类号：S432.42

摘要：为筛选对兰花枯萎病病原菌尖孢镰刀菌具有拮抗作用的芽孢细菌,从各地采集的土样中分离获得的237株芽孢杆菌进行初筛、复筛,最终从衡水饶阳县棉田的土样中筛选出的芽孢杆菌3A3-15,对病原菌有较强的抑菌活性,且抑菌谱广。结合形态学观察、生理生化试验,根据16S rDNA序列相似性分析,此菌株与Bacillus velezensis标准菌株CR-502的16S rDNA序列相似度达99.92%,因此,鉴定拮抗菌株3A3-15为Bacillus velezensis。
In order to obtain antagonistic bacteria against Fusarium oxysporium,237 bacillus strains were isolated and screened from soil samples of different districts.A bacillus strain 3A3-15 collected from cotton soil of Raoyang County in Hengshui was obtained via preliminary screening and secondary screening.It had strong and broad-spectrum inhibition capability.We characterized the strain 3A3-15 by morphological and culture features observation,physiological and biochemical experiments,and 16S rDNA sequence analysis.As the similarity of the 16S rDNA sequences between strain 3A3-15 and the type strain CR-502 was up to 99.92%,the strain 3A3-15 was finally identified as Bacillus velezensis.