文献类型：期刊文献

英文题名：Integrated metabolome and transcriptome analysis reveals the cause of anthocyanin biosynthesis deficiency in litchi aril

作者：Wang, Dan[1] Chen, Lei[1] Yang, Yabing[1] Abbas, Farhat[1] Qin, Yaqi[1] Lu, Hanle[1] Lai, Biao[2] Wu, Zichen[1] Hu, Bing[3] Qin, Yonghua[1] Wang, Huicong[1] Zhao, Jietang[1,4] Hu, Guibing[1,4]

第一作者：Wang, Dan

通信作者：Zhao, JT[1];Hu, GB[1]

机构：[1]South China Agr Univ, Coll Hort, Guangdong Litchi Engn Res Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Peoples R China;[2]Yangtze Normal Univ, Sch Adv Agr & Bioengn, Fuling, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab Trop Forestry Res, Natl Forestry & Grassland Adm, Guangzhou, Guangdong, Peoples R China;[4]South China Agr Univ, Coll Hort, Guangdong Litchi Engn Res Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510000, Peoples R China

年份：2023

卷号：175

期号：1

外文期刊名：PHYSIOLOGIA PLANTARUM

收录：;Scopus(收录号：2-s2.0-85148589681);WOS:【SCI-EXPANDED(收录号:WOS:000937261700022)】；

基金：China Litchi and Longan Industry Technology Research System, Grant/Award Number: CARS-32-05; Key Areas of Science and Technology Planning Project of Guangdong Province, Grant/Award Number: 2018B020202011; National Key Research and Development Program, Grant/Award Number: 2019FYD1000900; National Natural Science Foundation of China, Grant/Award Numbers: 31872066, 32272663; Science and Technology Planning Project of Guangzhou, Grant/Award Number: 202103000057; Science and Technology Talents and Platform Plan of Yunnan Province, Grant/Award Number: 202104AC100001-B04

语种：英文

摘要：Anthocyanins are health-promoting compounds with strong antioxidant properties that play important roles in disease prevention. Litchi chinensis Sonn. is a well-known and economically significant fruit due to its appealing appearance and nutritional value. The mature pericarp of litchi is rich in anthocyanins, whereas the aril (flesh) has an extremely low anthocyanin content. However, the mechanism of anthocyanin differential accumulation in litchi pericarp and aril remained unknown. Here, metabolome and transcriptome analysis were performed to unveil the cause of the deficiency of anthocyanin biosynthesis in litchi aril. Numerous anthocyanin biosynthesis-related metabolites and their derivatives were found in the aril, and the levels of rutin and (-)-epicatechin in the aril were comparable to those found in the pericarp, while anthocyanin levels were negligible. This suggests that the biosynthetic pathway from phenylalanine to cyanidin was present but that a block in cyanidin glycosylation could result in extremely low anthocyanin accumulation in the aril. Furthermore, 54 candidate genes were screened using weighted gene co-expression network analysis (WGCNA), and 9 genes (LcUFGT1, LcGST1, LcMYB1, LcSGR, LcCYP75B1, LcMATE, LcTPP, LcSWEET10, and LcERF61) might play a significant role in regulating anthocyanin biosynthesis. The dual-luciferase reporter (DLR) assay revealed that LcMYB1 strongly activated the promoters of LcUFGT1, LcGST4, and LcSWEET10. The results imply that LcMYB1 is the primary qualitative gene responsible for the deficiency of anthocyanin biosynthesis in litchi aril, which was confirmed by a transient transformation assay. Our findings shed light on the molecular mechanisms underlying tissue-specific anthocyanin accumulation and will help developing new red-fleshed litchi germplasm.