文献类型：期刊文献

英文题名：Insight into a highly polymorphic endophyte isolated from the roots of the halophytic seepweed Suaeda salsa: Laburnicola rhizohalophila sp. nov. (Didymosphaeriaceae, Pleosporales)

作者：Yuan, Zhilin[1,2] Druzhinina, Irina S.[3] Wang, Xinyu[2] Zhang, Xiaoguo[2] Peng, Long[1,2] Labbe, Jessy[4]

第一作者：袁志林

通信作者：Yuan, ZL[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou, Peoples R China;[3]Nanjing Agr Univ, Coll Resources & Environm Sci, Fungal Genom Grp, Nanjing, Peoples R China;[4]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA

年份：2020

卷号：124

期号：5

起止页码：327-337

外文期刊名：FUNGAL BIOLOGY

收录：;Scopus(收录号：2-s2.0-85074399284);WOS:【SCI-EXPANDED(收录号:WOS:000532395600010)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 31722014). JL was supported by the Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research as part of the PlanteMicrobe Interfaces Scientific Focus Area (http://pmi.ornl.gov).Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy (DOI:10.1038/s41598-017-00400-8 13) under contract DE-AC05-00OR22725. This article is part of the Fungal Adaptation to Hostile Challenges special issue for the third International Symposium on Fungal Stress (ISFUS), which is supported by the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (No. 2018/20571-6) and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (No. 88881.289327/2018-01).

语种：英文

外文关键词：Carbon utilization; Dark septate endophytes; Intraspecific polymorphism; Root-fungal mutualism

摘要：We surveyed root endophytic fungi of the coastal halophyte Suaeda salsa and detected a population of a novel species that we described here as Laburnicola rhizohalophila sp. nov. No sexual sporulating structure was observed. Instead, it produced a large amount of thalloconidia, 0-1 transverse septa, hyaline to darkly pigmented, often peanut-shaped and sometimes dumbbell-shaped, both ends enlarged with numerous oil droplets inside the hyphal cells. Surprisingly, a high degree of phenotypic and physiological intraspecific variation (e.g., salinity tolerance, growth under different carbon:nitrogen ratios, and carbon utilization pattern) was recorded. The inoculation test indicated that the isolates could successfully infect host roots and form microsclerotia-like structures in cortical cells, a typical trait of dark septate endophytes (DSEs). Furthermore, most isolates were shown to promote host seedling growth. To evaluate conspecificity and infer its phylogenetic affinity, multiloci data including nuclear rRNA loci (ITS1 and 2, partial 28S), partial RNA Polymerase II second-largest subunit (rpb2), and partial translation elongation factor-1 alpha (tef1) were characterized. Genealogical concordance phylogenetic species recognition (GCPSR) detected a genetically isolated Glade of L rhizohalophila within the Pleosporales in the Didymosphaeriaceae. Maximum likelihood phylogenetic reconstruction revealed that the endophytic fungus was genetically close to Laburnicola dactylidis but separated by a relatively long genetic distance. Our work highlights that the pleosporalean taxa might represent an underexplored reservoir of root DSEs. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.