文献类型：期刊文献

英文题名：Novel R2R3-MYB Transcription Factor LiMYB75 Enhances Leaf Callus Regeneration Efficiency in Lagerstroemia indica

作者：Jiang, Shengji[1] Lv, Fenni[1] Gao, Lulu[1] Gu, Jiaojiao[1,2] Yang, Rutong[1] Li, Sumei[1] Li, Ya[1] Li, Shaofeng[3] Wang, Peng[1,2]

第一作者：Jiang, Shengji

机构：[1] Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing, 210014, China; [2] College of Forestry, Nanjing Forestry University, Nanjing, 210037, China; [3] State Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing, 100091, China

年份：2023

卷号：14

期号：3

外文期刊名：Forests

收录：EI(收录号：20231513863445);Scopus(收录号：2-s2.0-85151712554)

语种：英文

外文关键词：Efficiency - Gene encoding - Plants (botany) - Transcription factors

摘要：Lagerstroemia indica is an important woody ornamental plant worldwide. However, the application of many technologies, such as transgenic breeding and genome editing, has been severely hampered due to the lack of efficient calli induction and regeneration technology. Here, we discussed a reliable and efficient calli induction and regeneration protocol using whole-leaf explants. This protocol’s effectiveness for the calli induction and regeneration systems in crape myrtle were up to 70.33% and 44.33%, respectively. Next, an efficient and stable Agrobacterium-mediated genetic transformation system was created from leaf calli, and the green fluorescent protein (GFP) was able to detect up to 90% of its positive frequency. Meanwhile, two positive lines’ transfer DNA insertion sites and directions were identified using whole genome sequencing. LiMYB75, a novel R2R3-MYB transcription factor, was identified and transferred to the L. indica genome to enhance the leaf calli regeneration frequency. Surprisingly, overexpressing LiMYB75 increased the frequency of calli regeneration in the leaf by 1.27 times and the number of regenerated plantlets per callus by 4.00 times compared to the wild type, by regulating the expression levels of genes involved in callus formation, such as SHOOT MERISTEMLESS (STM). Overall, our findings revealed a simple, reliable, and highly efficient transformation approach and identified the desirable candidate gene LiMYB75, which improves L. indica’s calli regeneration efficiency. ? 2023 by the authors.