文献类型：期刊文献

英文题名：Comparative Analyses of Five Complete Chloroplast Genomes from the Genus Pterocarpus (Fabacaeae)

作者：Hong, Zhou[1] Wu, Zhiqiang[2] Zhao, Kunkun[1] Yang, Zengjiang[1] Zhang, Ningnan[1] Guo, Junyu[1] Tembrock, Luke R.[3] Xu, Daping[1]

第一作者：洪舟

通信作者：Xu, DP[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Peoples R China;[2]Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr, Shenzhen 518120, Peoples R China;[3]Colorado State Univ, Dept Agr Biol, Ft Collins, CO 80523 USA

年份：2020

卷号：21

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000543400300020)】；

基金：This study was co-supported by the Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2020SZ005, CAFYBB2018SZ002) and National Natural Science Foundation of China (31500537).

语种：英文

外文关键词：Pterocarpus; chloroplast genome; hypervariable regions; microsatellite markers; purifying selection; phylogenetic analysis

摘要：Pterocarpus is a genus of trees mainly distributed in tropical Asia, Africa, and South America. Some species of Pterocarpus are rosewood tree species, having important economic value for timber, and for some species, medicinal value as well. Up to now, information about this genus with regard to the genomic characteristics of the chloroplasts has been limited. Based on a combination of next-generation sequencing (Illumina Hiseq) and long-read sequencing (PacBio), the whole chloroplast genomes (cp genomes) of five species (rosewoods) in Pterocarpus (Pterocarpus macrocarpus, P. santalinus, P. indicus, P. pedatus, P. marsupium) have been assembled. The cp genomes of five species in Pterocarpus have similar structural characteristics, gene content, and sequence to other flowering plants. The cp genomes have a typical four-part structure, containing 110 unique genes (77 protein coding genes, 4 rRNAs, 29 tRNAs). Through comparative genomic analysis, abundant simple sequence repeat (SSR)loci (333-349) were detected in Pterocarpus, among which A /T single nucleotide repeats accounted for the highest proportion (72.8-76.4%). In the five cp genomes of Pterocarpus, eight hypervariable regions, including trnH-GUG_psbA, trnS-UGA_psbC, accD-psaI, ndhI-exon2_ndhI-exon1, ndhG_ndhi-exon2, rpoC2-exon2, ccsA, and trnfM-CAU, are proposed for use as DNA barcode regions. In the comparison of gene selection pressures (P. santalinus as the reference genome), purifying selection was inferred as the primary mode of selection in maintaining important biological functions. Phylogenetic analysis shows that Pterocarpus is a monophyletic group. The species P. tinctorius is resolved as early diverging in the genus. Pterocarpus was resolved as sister to the genus Tipuana.