文献类型：期刊文献

英文题名：Physiological and transcriptional responses of Catalpa bungei to drought stress under sufficient- and deficient-nitrogen conditions

作者：Shi, Huili[1] Ma, Wenjun[2] Song, Junyu[1] Lu, Mei[1] Rahman, Siddiq Ur[3] Thi Tuyet Xuan Bui[1] Dinh Duy Vu[1] Zheng, Huifang[1] Wang, Junhui[2] Zhang, Yi[1]

第一作者：Shi, Huili

通信作者：Zhang, Y[1];Wang, JH[2]

机构：[1]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China

年份：2017

卷号：37

期号：11

起止页码：1457-1468

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000416400500002)】；

基金：This research was jointly supported by the Start-up Project for Introduced Recruit in Northwest A&F University (No. Z111021402), the 12th Five-Year Plan of National Science and Technology Support of China (No. 2012BAD21B03), the National Natural Science Foundation of China (No. 31370671) and the Fundamental Research Funds for the Central Universities (No. Z109021566).

语种：英文

外文关键词：Catalpa bungei; drought resistance; gene expression; nitrogen application; nitrogen deficiency

摘要：Many semi-arid ecosystems are simultaneously limited by soil water and nitrogen (N). We conducted a greenhouse experiment to address how N availability impacts drought-resistant traits of Catalpa bungei C. A. Mey at the physiological and molecular level. A factorial design was used, consisting of sufficient-N and deficient-N combined with moderate drought and well-watered conditions. Seedling biomass and major root parameters were significantly suppressed by drought under the deficient-N condition, whereas N application mitigated the inhibiting effects of drought on root growth, particularly that of fine roots with a diameter <0.2 mm. Intrinsic water-use efficiency was promoted by N addition under both water conditions, whereas stable carbon isotope compositions (delta C-13) was promoted by N addition only under the well-watered condition. Nitrogen application positively impacted drought adaptive responses including osmotic adjustment and homeostasis of reactive oxygen species, the content of free proline, soluble sugar and superoxide dismutase activity: all were increased upon drought under sufficient-N conditions but not under deficient-N conditions. The extent of abscisic acid (ABA) inducement upon drought was elevated by N application. Furthermore, an N-dependent crosstalk between ABA, jasmonic acid and indole acetic acid at the biosynthesis level contributed to better drought acclimation. Moreover, the transcriptional level of most genes responsible for the ABA signal transduction pathway, and genes encoding the antioxidant enzymes and plasma membrane intrinsic proteins, are elevated upon drought only under sufficient-N addition. These observations confirmed at the molecular level that major adaptive responses to drought are dependent on sufficient N nutrition. Although N uptake was decreased under drought, N-use efficiency and transcription of most genes encoding N metabolism enzymes were elevated, demonstrating that active N metabolism positively contributed drought resistance and growth of C. bungei under sufficient-N conditions.