文献类型：期刊文献

英文题名：The Larix kaempferi genome reveals new insights into wood properties

作者：Sun, Chao[1,2] Xie, Yun-Hui[1,2] Li, Zhen[3,4] Liu, Yan-Jing[1] Sun, Xiao-Mei[1,2] Li, Jing-Jing[5] Quan, Wei-Peng[5] Zeng, Qing-Yin[1] van de Peer, Yves[3,4,6] Zhang, Shou-Gong[1,2]

第一作者：孙超

通信作者：Zeng, QY[1];Zhang, SG[1];Zhang, SG[2];van de Peer, Y[3];van de Peer, Y[4];van de Peer, Y[5]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium;[4]VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium;[5]Next Biosci Co Ltd, Wuhan 430073, Peoples R China;[6]Dept Biochem Genet & Microbiol, Pretoria, South Africa

年份：2022

卷号：64

期号：7

起止页码：1364-1373

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000822742000009)】；

基金：We acknowledge Dr. Shae He, Dr. Leiming Dong, and Dr. Weibo Xiang for their help in preparing sequencing samples. We also thank Ruixue Li for her help in data analysis. This work was funded by the Forestry Industry Research Special Funds for Public Welfare Projects (201504104), National Natural Science Foundation of China (31901335), Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2017QA001, CAFYBB2018ZY001-4, LYSZX202002), and National Transgenic Major Program (2018ZX08020-003). Z.L. is funded by a postdoctoral fellowship from the Special Research Fund of Ghent University (BOFPDO2018001701).

语种：英文

外文关键词：evolution; gene expression; genome; Larix kaempferi; wood properties

摘要：Here, through single-molecule real-time sequencing, we present a high-quality genome sequence of the Japanese larch (Larix kaempferi), a conifer species with great value for wood production and ecological afforestation. The assembled genome is 10.97 Gb in size, harboring 45,828 protein-coding genes. Of the genome, 66.8% consists of repeat sequences, of which long terminal repeat retrotransposons are dominant and make up 69.86%. We find that tandem duplications have been responsible for the expansion of genes involved in transcriptional regulation and stress responses, unveiling their crucial roles in adaptive evolution. Population transcriptome analysis reveals that lignin content in L. kaempferi is mainly determined by the process of monolignol polymerization. The expression values of six genes (LkCOMT7, LkCOMT8, LkLAC23, LkLAC102, LkPRX148, and LkPRX166) have significantly positive correlations with lignin content. These results indicated that the increased expression of these six genes might be responsible for the high lignin content of the larches' wood. Overall, this study provides new genome resources for investigating the evolution and biological function of conifer trees, and also offers new insights into wood properties of larches.