文献类型：期刊文献

英文题名：Dual-functional antimicrobial coating based on a quaternary ammonium salt from rosin acid with in vitro and in vivo antimicrobial and antifouling properties

作者：Li, Zhaoshuang[1,2] Yang, Xinxin[1] Liu, He[1] Yang, Xiaohui[1] Shan, Yu[3,4] Xu, Xu[2] Shang, Shibin[1] Song, Zhanqian[1]

第一作者：Li, Zhaoshuang

通信作者：Liu, H[1];Song, Z[1]|[a0005c3716bb5fed5ea85]宋湛谦;

机构：[1]Chinese Acad Forestry, State Forestry Adm, Inst Chem Ind Forestry Prod,Key & Open Lab Forest, Natl Engn Lab Biomass Chem Utilizat,Key Lab Bioma, Nanjing 210042, Jiangsu, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Jiangsu Prov Key Lab Chem & Utilizat Agroforest B, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China;[3]Inst Bot, Nanjing, Jiangsu, Peoples R China;[4]Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China

年份：2019

卷号：374

起止页码：564-575

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20192307008142);Scopus(收录号：2-s2.0-85066461890);WOS:【SCI-EXPANDED(收录号:WOS:000477652900051)】；

基金：The authors express their gratitude for financial support from the National Natural Science Foundation of China (31570562) and the Foundation of Jiangsu Province Biomass Energy and Material Laboratory (JSBEM-S-201905).

语种：英文

外文关键词：Rosin acid-derived quaternary ammonium; Dual-functional coating; Antimicrobial; Antifouling; Biocompatibility

摘要：Bacterial colonization and biofilm formation on indwelling medical devices potentially cause serious and life-threatening infections. Technologies to prevent bacterial colonization and biofilm formation on biomedical devices are in great demand. Here, we report the preparation of coating monomers by conjugating rosin acid-derived maleopimaric acid quaternary ammonium cation (MPA-N+) with different chain of lengths allyloxy polyethylene glycol (APEG; either MW 1200 or 2400). The monomers of the cation plus its linker conjugates (APEG(1200/2400)-MPA-N+) were further grafted onto the surface of silicone rubber slides (PDMS) via plasma/UV-induced surface polymerization to form antimicrobial and antifouling coatings. The dual-functional coatings exhibited excellent antimicrobial activities against gram-negative and positive bacteria, and they effectively prevented biofilm formation. The coatings also greatly reduced protein adsorption and platelet adhesion, indicating their excellent antifouling capability. In addition, the APEG(1200/2400)-MPA-N+ cation coatings were biocompatible towards mammalian cells, as shown by in vitro cytotoxicity assays using rabbit erythrocytes and attached human aorta smooth muscle cells. In addition, the APEG(2400)-MPA-N+ coating exhibited significant anti-infective activity in a rodent subcutaneous infection model. We show that the APEG(2400)-MPA-N+ coating is an effective strategy for the prevention of infections associated with indwelling biomedical devices.