文献类型：期刊文献

中文题名：白皮松天然群体遗传多样性的EST-SSR分析

英文题名：Genetic Diversity Analysis of Pinus bungeana Natural Populations with EST-SSR Markers

作者：赵罕[1,2] 郑勇奇[1] 李斌[1] 张川红[1] 林富荣[1] 于雪丹[1] 程蓓蓓[1] 黄平[1]

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室;[2]中国林业科学研究院经济林研究开发中心

年份：2014

卷号：27

期号：4

起止页码：474-480

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家科技支撑计划课题(2013BAD01B06)

语种：中文

中文关键词：白皮松;天然群体;遗传多样性

外文关键词：Pinus bungeana ; natural population ; EST-SSR ; genetic diversity

分类号：S791.243

摘要：为探讨白皮松群体间遗传变异规律,使用7对EST-SSR引物对分布区内21个白皮松天然群体的遗传多样性及遗传分化水平进行了研究。结果表明:7对引物在21个白皮松天然群体的663个单株中共检测到14个多态性位点。各群体间有效等位基因数(Ne)、Shannon’s信息指数(I)、观测杂合度(Ho)、期望杂合度(He)、Nei’s期望杂合度(Nei’s)分别为1.156 5 1.601 9、0.133 5 0.492 5、0.138 4 0.397 3、0.0860 0.342 8、0.084 6 0.337 4。白皮松群体间遗传分化系数(Fst)平均为0.215 2,基因流(Nm)值平均为0.911 9,群体间基因交流总体较少,遗传分化较大。白皮松多样性水平在分布区内呈规律性变化,多样性分布的中心区域主要在西部、南部,具有从西向东,从南向北依次减少的趋势。
In order to explore the genetic variation among Pinus bungeana populations, their genetic diversity and differentiation level were studied in 21 natural populations by EST-SSR markers. 14 polymorphic loci were detected in 663 individuals among 21 natural populations using 7 pairs of EST-SSR primes. The effective number of alleles （ Ne）, Shannon＇ s information index （ I）, observed heterozygosity （ Ho）, expected heterozygosity （He） and Nei＇ s expected heterozygosity （Nei＇ s） were 1. 156 5--1. 601 9, 0. 133 5---0. 492 5, 0. 138 4---0. 397 3, 0. 086-- 0.342 8, and 0.084 6-----0.337 4 respectively. The results of genetic differentiation analysis showed that the average Fst and Nm were 0.215 2 and 0.911 9 respectively, the gene flow was less and the genetic differentiation was high among populations. Central regions of genetic diversity are mainly in west and south of the distribution and the genetic diversity in distribution changed regularly from west to east and from south to north.