文献类型：期刊文献

英文题名：Genome-Wide Investigation of the CBL-CIPK Gene Family in Oil Persimmon: Evolution, Function and Expression Analysis during Development and Stress

作者：Liu, Cuiyu[1,2] Wang, Yanpeng[1,2] Yao, Jin[3] Yang, Xu[1,2] Wu, Kaiyun[1,2] Teng, Guoxin[3] Gong, Bangchu[1,2] Xu, Yang[1,2]

第一作者：Liu, Cuiyu;刘翠玉

通信作者：Gong, BC[1];Xu, Y[1];Gong, BC[2];Xu, Y[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou, Peoples R China;[3]Lanxi City Nursery Zhejiang Provence, Lanxi 321100, Peoples R China

年份：2023

卷号：9

期号：1

外文期刊名：HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-85146810681);WOS:【SCI-EXPANDED(收录号:WOS:000914589900001)】；

基金：This research was funded by the National Key R & D Program of China (grant No. 2019YFD1000600 and 2018YFD1000606), and the Key Agricultural New Varieties Breeding Projects of the Zhejiang Province Science and Technology Department (grant No. 2021C02066-10).

语种：英文

外文关键词：oil persimmon; CBL-CIPK genes; evolution analysis; expression pattern; subcellular location

摘要：Ca2+-sensors, calcineurin B-like proteins (CBLs), and calcineurin B-like protein-interacting protein kinases (CIPKs) form a CBL-CIPK complex to regulate signal transduction. This study aimed to reveal the characteristics of the CBL-CIPK gene family in oil persimmon (Diospyros oleifera). Ten DoCBL and 23 DoCIPK genes were identified, and gene duplication among them was mainly attributed to segmental duplication. According to phylogenetic and structural analysis, DoCBLs were clustered into four groups with distinct motifs, namely myristoylation and palmytoylation sites in their N-terminus, and DoCIPKs containing a NAF/FISL domain were clustered into intron-rich and intron-less groups. The expression patterns of DoCBLs and DoCIPKs were tissue- and time-specific in different tissues and at different stages of fruit development. Most CBL-CIPK genes were upregulated under NaCl, drought, and Ca(NO3)(2) stress using qRT-PCR analysis. DoCBL5 and DoCIPK05 were both located in the plasma membrane of cells using green fusion proteins (GFP) in tobacco leaves. DoCBL5 and DoCIPK05 might interact with AKT1, PP2C, and SNF to regulate the Ca2+ signals, K+, and ABA homeostasis in cells. In conclusion, these results suggested that the CBL-CIPK family genes might play important roles in oil persimmon growth and stress responses.