文献类型：期刊文献

中文题名：云杉花墨天牛成虫携带松材线虫数量的研究

英文题名：Study on the number of Bursaphelenchus xylophilus carried by Monochamus saltuarius adult

作者：王珏[1] 张彦龙[2] 舒红[3] 郑雅楠[1]

第一作者：王珏

机构：[1]沈阳农业大学林学院,辽宁沈阳110866;[2]中国林业科学研究院森林生态环境与自然保护研究所/国家林业和草原局森林保护学重点实验室,北京100091;[3]辽宁省林业有害生物防治检疫站,辽宁沈阳110804

年份：2023

卷号：45

期号：8

起止页码：142-147

中文期刊名：北京林业大学学报

外文期刊名：Journal of Beijing Forestry University

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：辽宁省应用基础研究计划项目(2023JH2/101300130)。

语种：中文

中文关键词：云杉花墨天牛;松材线虫;携带量;媒介昆虫

外文关键词：Monochamus saltuarius;Bursaphelenchus xylophilus;carrying amount;vector insect

分类号：S763.3

摘要：【目的】媒介昆虫携带松材线虫的数量对松材线虫的传播危害有着重要影响,明确我国松材线虫媒介昆虫云杉花墨天牛携带松材线虫的数量,对松材线虫防治具有重要意义。【方法】本研究于2018-2020年在辽宁省抚顺市大伙房实验林场开展了调查。采集被云杉花墨天牛危害的红松疫木,锯成1 m长木段,两端蜡封保湿,置于养虫笼内饲养,每天收集初羽化的云杉花墨天牛。同时通过悬挂诱捕器和震落收集感病红松林内的云杉花墨天牛并带回实验室。将所有收集到的云杉花墨天牛活体解剖为头、胸、腹3部分,采用贝曼式漏斗法分离其体内携带的线虫,并记录松材线虫数量。【结果】结果表明:疫木包网中收集的160头云杉花墨天牛初羽化成虫体内携带线虫的天牛有58头,占总体的36.25%。平均每头天牛携带松材线虫336.74条,携带量最多为5560条,雌成虫平均携带量为458.52条,雄成虫平均携带量为191.54条,雌雄差异不显著;林间收集的274头云杉花墨天牛成虫体内携带线虫的天牛有38头,占总体的13.87%。平均每头天牛携带线虫214.92条,携带量最多为2486条,雌成虫平均携带量为161.81条,雄成虫平均携带量为280.53条,雌雄差异不显著;云杉花墨天牛胸部线虫数量最多,平均为35.88条,其次是头部,平均为0.88条,最少的是腹部,平均为0.34条,胸部线虫数量显著高于其他两个部分的线虫数量。【结论】本研究发现我国云杉花墨天牛成虫体内松材线虫携带量远低于日本云杉花墨天牛,更低于松褐天牛的携带量,雌雄天牛成虫间携带量差异不显著,且松材线虫主要分布于天牛胸部。
[Objective]The amount of Bursaphelenchus xylophilus carried by vector insects has an important impact on the transmission and damage of pine wilt disease,so it is necessary to clarify the carrying amount of Monochamus saltuarius vectoring B.xylophilus in China.[Method]The number of B.xylophilus carried by M.saltuarius was investigated in Dahuofang Experimental Forest Farm in Fushun City,Liaoning Province of northeastern China in 2018 to 2020.Pinus koraiensis trees infected by M.saltuarius were sawn into one meter long logs,sealed with wax at both ends to retain moisture,and placed in cages.The newly emerged M.saltuarius was collected every day.Meanwhile,the M.saltuarius was collected from infected P.koraiensis by hanging sentinel traps and shock-dropping methods,and brought to the laboratory.All the collected live M.saltuarius trees were dissected into three parts:head,thorax and abdomen to separate nematodes by the Bayman funnel method,then the number of B.xylophilus was counted.[Result]58 vectors were tested carrying B.xylophilus among 160 newly emerged adults of M.saltuarius,accounting for 36.25%of the total.The average number of B.xylophilus carried by each M.saltuarius was 336.74,and the maximum number was 5560.The average number of B.xylophilus carried by each female adult and male adult was 458.52 and 191.54,respectively,with no significant difference between male and female.38 vectors were tested carrying B.xylophilus among 274 M.saltuarius adults collected in forest,accounting for 13.87%of the total.The average number of B.xylophilus carried by each M.saltuarius was 214.92,and the maximum number was 2486.The average number of B.xylophilus carried by each female adult and male adult was 161.81 and 280.53,respectively,with no significant difference between male and female.The average number of B.xylophilus in thorax,head,and abdomen of M.saltuarius was 35.88,0.88,and 0.34,respectively.The number of B.xylophilus in vector’s thorax was much higher than that in the other two parts.[Conclusion]It is found that the number of B.xylophilus carried by M.saltuarius in China was much lower than M.saltuarius in Japan and M.alternatus in this study.There was no significant difference in the carrying amount between male and female adults of M.saltuariu,and B.xylophilus was mainly distributed in the thorax of the vector.