文献类型：期刊文献

英文题名：CjPLE, a PLENA-like gene, is a potential regulator of fruit development via activating the FRUITFUL homolog in Camellia

作者：Lyu, Tao[1,2,3] Fan, Zhengqi[1,2] Yang, Wen[1,2] Yan, Chao[1,2,4] Hu, Zhikang[1,2] Li, Xinlei[1,2] Li, Jiyuan[1,2] Yin, Hengfu[1,2]

第一作者：Lyu, Tao

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Ningbo Univ, Coll Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China

年份：2019

卷号：70

期号：12

起止页码：3153-3164

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000484414200013)】；

基金：We thank the anonymous reviewer and Dr Rainer Melzer (University College Dublin) for critical comments that helped to improve the manuscript. This work was supported by Nonprofit Research Projects (CAFYBB2017ZX001-7, CAFYBB2016SZ001) of Chinese Academy of Forestry, and National Science Foundation of China (NSFC Grant 31870578). The authors are grateful to Prof. Zhongchi Liu from University of Maryland for the help of chromatin immunoprecipitation experiments.

语种：英文

外文关键词：Camellia; chromatin immunoprecipitation; fruit development; FRUITFUL; MADS-box gene; PLENA

摘要：Fruit patterning involves the cooperation of multiple processes, including metabolic change, cell differentiation, and cell expansion. The FRUITFUL (FUL) and SHATTERPROOF1/2 (SHPs) MADS-box genes are master regulators directing fruit patterning in several eudicots. However, the regulatory mechanisms of the FUL-SHP network in different fruit types remain unclear. Here, we characterized the functions of an ortholog (CjPLE) of SHPs from Camellia japonica. We showed that CjPLE was predominantly expressed in stamen and carpel tissues during the early stage of floral development and that transcripts were abundant in the pericarp tissues during fruit development. The ectopic expression of CjPLE in Arabidopsis caused enhanced development of the carpels, whereas no defects in floral identity were observed. To investigate the downstream targets of CjPLE, overexpression transformants were analysed through a callus transformation system in Camellia azalea. We examined the expression levels of potential downstream target genes and found that two previously identified APETALA1-like genes (CjAPL1/2) were significantly up-regulated. We showed that CjPLE directly bound to the CArG motifs in the promoter region of CjAPL1 (the FUL ortholog). Taken together, our results reveal a possible positive regulation of FUL by SHP in the control of fruit development in Camellia.