文献类型：期刊文献

英文题名：The developmental transcriptome dynamics of current-year shoot utilized as scion in Camellia chekiangoleosa

作者：Sheng, Yu[1,3] Gao, Haili[2] Yu, Chunlian[3] Huang, Guangyuan[3] Wang, Kunxi[3] Wang, Kailiang[1] Lv, Leyan[4] Long, Wei[1]

第一作者：盛宇;Sheng, Yu

通信作者：Long, W[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Peoples R China;[2]Zhejiang Publ Welf Forest & State Forest Farm Mana, Hangzhou 310019, Zhejiang, Peoples R China;[3]Changshan Country Oil Tea Ind Dev Ctr, Quzhou Doctoral Innovat Workstn, Quzhou 323900, Peoples R China;[4]Zhejiang Tongji Vocat Coll Sci & Technol, Coll Hydraul Engn, Hangzhou 311231, Zhejiang, Peoples R China

年份：2025

卷号：25

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-105006828313);WOS:【SCI-EXPANDED(收录号:WOS:001497469700001)】；

基金：We appreciate Ph.D. student Linxiu Liu for the operation of qRT-PCR.

语种：英文

外文关键词：Transcriptome analysis; Shoot development; Camellia chekiangoleosa

摘要：BackgroundCamellia chekiangoleosa is the most widely planted red-flowered and large-fruited oil-camellia species, with high value in edible oil production and landscaping. To better understand the weak scion development and slow graft-union healing underlying grafting propagation challenges in C. chekiangoleosa, we conducted temporal RNA-seq on current-year shoots with five time points determined according to changes in cell wall composition, aiming to reveal dynamic developmental regulation.ResultsAnalysis of temporal expression characteristics of genome-wide genes and differentially expressed genes (DEGs) revealed that genes differentially patterned between stem and apical bud were enriched in functions related to cell division and differentiation, hormone responses, and vascular or flower development. Coexpression network analysis revealed that red/far-red light and gibberellin (GA) signaling were closely correlated with flowering development in C. chekiangoleosa shoots. We further analyzed a unique module showing a negative correlation between the module and traits (cell wall composition, i.e., lignin, cellulose, and hemicellulose content). Genes in the top-scored sub-cluster of this module were enriched in shoot development-related processes, including cell wall dynamics, xylem development, secondary cell wall biogenesis, lignin biosynthesis, and procambium histogenesis. WOX4 and PXY, known markers of cambium cells, were identified as key hub genes, along with the actin-binding protein WLIM1. These coexpressed hub genes associated with vascular system development peaked in June in stems and were validated by qRT-PCR, suggesting that June may be an optimal grafting season for C. chekiangoleosa.ConclusionsIntegrating transcriptomics and physiology defined the dynamic signature of changes in composition of cell wall and gene activity during the development of current-year shoots in C. chekiangoleosa. Our findings provide insights into a potential molecular strategy for breeders, targeting key regulators specific to cambium differentiation, and physiological strategy for hormone or light supplied artificially to enhance grafting productivity of C. chekiangoleosa.