文献类型：期刊文献

英文题名：Integrated study on subcellular localization and chemical speciation of Pb reveals root strategies for Pb sequestration and detoxification in Salix integra

作者：Wang, Shufeng[1] Shi, Xiang[1] Salam, Mir Md Abdus[2] Chen, Guangcai[1]

第一作者：王树凤

通信作者：Chen, GC[1]|[a000551c9abd7c3960d59]陈光才;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Univ Eastern Finland, Sch Forest Sci, Joensuu 80100, Finland

年份：2021

卷号：467

期号：1-2

起止页码：197-211

外文期刊名：PLANT AND SOIL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000685726400001)】；

基金：This work was supported by the National Natural Science Foundation of China (31770653; 314000526) and the Zhejiang Science and Technology Major Program on Agricultural (trees) New Variety Breeding (2016C02056-11). We acknowledge the support from the 4W1B beamline of BSRF (Beijing Synchrotron Radiation Facility), Institute of High Energy Physics, Chinese Academy of Sciences. We are grately thankful for the help of Dr. Dongliang Chen and Dr. Juncai Dongfor their technical assistance in data acquisition and processing. We are also thankful to Dr. Hengfu Yin and Dr. Xiaojiao Han for their valuable suggestions during the revision period.

语种：英文

外文关键词：Pb; Phytostablization; Subcellular distribution; Phytosequestration

摘要：Background and aims Salix integra has a high tolerance to lead (Pb) stress, and has been applied in the phytostabilization of Pb-Zn mine tailings in East China. Mechanisms for Pb sequestration and detoxification were poorly understood in Salix plants. The present study aimed to elucidate where Pb was localized and how Pb was combined in roots of S. integra for a better understanding of Pb tolerance strategies. Methods Histochemical methods combined with synchrotron-based X-ray fluorescence microscopy, transmission electron microscopy and fourier transform infrared spectroscopy were used to explore the distribution and chemical forms of Pb at cellular and subcellular levels in S. integra. Results The accumulation of Pb displayed a fast linear uptake in roots and stems in the short-term period, whereas a slower Freundlich-like uptake in leaves. Micro-XRF and histochemical staining revealed that the absorbed Pb in roots was mostly restricted in the cortex, and partly translocated in the stele. At the subcellular level, most Pb in roots was localized in the cell walls and trophoplast fraction, and the free -OH and pectin C-O/C-O-S groups may be involved in the cell wall sequestration. It was also found that the majority of Pb in roots existed as phosphate and oxalate. Conclusions S. integra has a high uptake potential of accumulating Pb in their roots. The absorbed Pb was primarily localized in the cell walls and combined with -OH or pectin groups, and precipitation as phosphate or oxalate may be responsible for the detoxification in root cells.