文献类型：期刊文献

英文题名：Dual roles of glutathione in silver nanoparticle detoxification and enhancement of nitrogen assimilation in soybean (Glycine max(L.) Merrill)

作者：Ma, Chuanxin[1,2] Liu, Hong[3] Chen, Guangcai[4] Zhao, Qing[5] Guo, Huiyuan[1] Minocha, Rakesh[6] Long, Stephanie[6] Tang, Yuanzhi[7] Saad, Emily M.[7] DeLaTorreRoche, Roberto[2] White, Jason C.[2] Xing, Baoshan[1] Dhankher, Om Parkash[1]

第一作者：Ma, Chuanxin

通信作者：Xing, BS[1];Dhankher, OP[1]

机构：[1]Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA;[2]Connecticut Agr Expt Stn, Dept Analyt Chem, New Haven, CT 06504 USA;[3]Fujian Agr & Forestry Univ, Coll Resources & Environm Sci, Fuzhou 350002, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[5]Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China;[6]Forest Serv, USDA, NRS, 271 Mast Rd, Durham, NH 03824 USA;[7]Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA

年份：2020

卷号：7

期号：7

起止页码：1954-1966

外文期刊名：ENVIRONMENTAL SCIENCE-NANO

收录：;EI(收录号：20203609124584);Scopus(收录号：2-s2.0-85090006444);WOS:【SCI-EXPANDED(收录号:WOS:000549099000003)】；

基金：This research was supported by the USDA-AFRI (2011-67006-30181) and the USDA-NIFA Hatch program (MAS 00549, MAS 00401, CONH 00147). Y. Tang acknowledges the funding support from Georgia Water Resource Institute. We appreciate the support from beamline scientists Drs. Mali Balasubramanian and Chengjun Sun at APS Beamline 20-ID-B and Dr. Qing Ma at APS Beamline 5-BM-D. Portions of this research were conducted at the Advanced Photon Source (APS). Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Office of Science and Office of Basic Energy Sciences. H. Liu acknowledges FAFU Technology and Innovation Project (KFA17149A).

语种：英文

外文关键词：Agricultural robots - Amino acids - Detoxification - Food supply - Histology - Metal nanoparticles - Nitrogen - Peptides - Silver nanoparticles - Soil pollution - Soils - Tissue - Tissue engineering - Toxicity

摘要：Widespread use of silver nanoparticles (Ag NPs) as pesticides and fungicides in agriculture is a major environmental concern. In the present study, soybean (Glycine maxL.) was grown in Ag NP (0-62.5 mg kg(-1))-amended soil with or without the addition of glutathione (GSH). Ag NPs exerted severe phytotoxicity and caused the reduction of shoot and root biomass and total number of nodules or completely inhibited nodule formation at doses above 31.25 mg kg(-1). Synchrotron-based techniques were applied to analyze Ag speciation in both the soil and the soybean root tissues at harvest. The results indicate that the majority of Ag remained in the form of Ag NPs and that 23% was present as Ag2S in soil; in both root and nodule tissues, Ag-GSH was the main component (40.6-88%) other than Ag NPs (12-59.4%), highlighting the important role of GSH in alleviating the Ag NP-induced toxicity. The addition of 0.8 mM GSH not only significantly increased fresh biomass by 85% in the 62.5 mg kg(-1)Ag NP treatment but also decreased Ag accumulation by 24.8-27% in soybean tissues. Although the addition of 0.8 mM GSH reduced the nodule number and weight as compared to the control, the total nitrogen content in soybean co-treated with Ag NP and GSH was more than 5-fold higher than that in the Ag NP alone treatments, suggesting that GSH may be utilized as a nitrogen source while simultaneously alleviating Ag NP toxicity. The shoot and root contents of thiol compounds (cysteine and gamma-glutamylcysteine) in the GSH treatments were several folds higher than that in the control and Ag NP alone treatments. Further, higher levels of essential amino acids, particularly alanine, glutamate, and glutamine which play important role in N assimilation in plants, in soybean across all the treatments further confirmed that GSH was utilized as a nitrogen source, resulting in enhanced soybean growth. Taken together, this study clearly demonstrated the negative impact of Ag NPs on soybean productivity and N fixation and highlights the protective role of GSH against Ag NP-induced toxicity. These findings have significant relevance for developing future strategies to minimize crop loss in marginal or contaminated soils, subsequently enhancing global food security.