文献类型：期刊文献

英文题名：DNA Barcoding Analysis and Phylogenetic Relation of Mangroves in Guangdong Province, China

作者：Wu, Feng[1,2] Li, Mei[1] Liao, Baowen[1] Shi, Xin[1] Xu, Yong[3,4]

第一作者：Wu, Feng

通信作者：Liao, BW[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry Adm Trop Forestry Res, Guangzhou 510520, Guangdong, Peoples R China;[2]Zhaoqing Xinghu Natl Wetland Pk Management Ctr, Zhaoqing 526060, Peoples R China;[3]Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China;[4]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2019

卷号：10

期号：1

外文期刊名：FORESTS

收录：;EI(收录号：20190306397711);Scopus(收录号：2-s2.0-85060027707);WOS:【SCI-EXPANDED(收录号:WOS:000458910800056)】；

基金：This research was funded by the Ministry of Science and Technology of China (No. 2017FY100705 and No. 2017FY100700), and the National Natural Science Foundation of China (No. 31570594).

语种：英文

外文关键词：mangroves; DNA barcoding; species identification; phylogenetic relation

摘要：Mangroves are distributed in the transition zone between sea and land, mostly in tropical and subtropical areas. They provide important ecosystem services and are therefore economically valuable. DNA barcoding is a useful tool for species identification and phylogenetic reconstruction. To evaluate the effectiveness of DNA barcoding in identifying mangrove species, we sampled 135 individuals representing 23 species, 22 genera, and 17 families from Zhanjiang, Shenzhen, Huizhou, and Shantou in the Guangdong province, China. We tested the universality of four DNA barcodes, namely rbcL, matK, trnH-psbA, and the internal transcribed spacer of nuclear ribosomal DNA (ITS), and examined their efficacy for species identification and the phylogenetic reconstruction of mangroves. The success rates for PCR amplification of rbcL, matK, trnH-psbA, and ITS were 100%, 80.29% +/- 8.48%, 99.38% +/- 1.25%, and 97.18% +/- 3.25%, respectively, and the rates of DNA sequencing were 100%, 75.04% +/- 6.26%, 94.57% +/- 5.06%, and 83.35% +/- 4.05%, respectively. These results suggest that both rbcL and trnH-psbA are universal in mangrove species from the Guangdong province. The highest success rate for species identification was 84.48% +/- 12.09% with trnH-psbA, followed by rbcL (82.16% +/- 9.68%), ITS (66.48% +/- 5.97%), and matK (65.09% +/- 6.00%), which increased to 91.25% +/- 9.78% with the addition of rbcL. Additionally, the identification rate of mangroves was not significantly different between rbcL + trnH-psbA and other random fragment combinations. In conclusion, rbcL and trnH-psbA were the most suitable DNA barcode fragments for species identification in mangrove plants. When the phylogenetic relationships were constructed with random fragment combinations, the optimal evolutionary tree with high supporting values (86.33% +/- 4.16%) was established using the combination of matK + rbcL + trnH-psbA + ITS in mangroves. In total, the 476 newly acquired sequences in this study lay the foundation for a DNA barcode database of mangroves.