文献类型：期刊文献

中文题名：土壤细菌对土壤中绿僵菌孢子萌发的影响

英文题名：Inhibition Effects of Soil Bacteria on Conidium Germination of Metarhizium pingshaense

作者：张亚波[1] 吴小双[1] 叶碧欢[1] 王浩杰[1] 舒金平[1]

第一作者：张亚波

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2017

卷号：33

期号：6

起止页码：788-795

中文期刊名：中国生物防治学报

外文期刊名：Chinese Journal of Biological Control

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：公益性行业(林业)科研专项(201304403);国家重点研发计划项目(2016YFD0600903)

语种：中文

中文关键词：平沙绿僵菌;土壤细菌;土壤因子;抗生素;16S;rDNA

外文关键词：Metarhizium pingshaense; soil bacterium; soil factors; antibiotics; 16S rDNA

分类号：S476.1

摘要：平沙绿僵菌Metarhizium pingshaense是一种潜力巨大的生防真菌,其能否在土壤中快速定殖是有效控制地下害虫的重要条件,而孢子萌发是定殖的第一步。本文研究了竹林覆盖地和非覆盖地2种土壤在灭菌和添加抗生素条件下对绿僵菌孢子萌发的影响,并利用16S rDNA基因序列测序方法进一步分析土壤细菌中影响平沙绿僵菌孢子萌发的细菌类群。结果表明,在覆盖地土壤中平沙绿僵菌孢子萌发率较高,与非覆盖地差异显著;土壤灭菌处理后,非覆盖地土壤中平沙绿僵菌孢子萌发率显著提高,与覆盖地孢子萌发率差异不显著。添加6种抗生素后,平沙绿僵菌孢子萌发率在非覆盖地土壤中差异显著,在覆盖地差异不显著。非覆盖地土壤中细菌总数及多样性均高于覆盖地土壤,其中酸杆菌门Acidobacteria、放线菌门Actinobacteria和芽单胞菌门Gemmatimonadetes显著高于覆盖地土壤。
Metarhizium pingshaense is a biological control agent with huge potential. It is an important prerequisite of effective control of soil dwelling pests whether the introduced M. pingshaense can establish rapidly in the soil, where conidium germination is the first and crucial step of colonization. In this research, conidium germination of M. pingshaense in soils from uncovered and covered bamboo garden was measured under different soil conditions, including soil sterilization and supplement of different antibiotics, then soil 16 S rDNA metagenomics was used to analyze the species structure of the soil bacterium. The results showed that conidium germination of M. pingshaense in soil from covered bamboo garden was significantly higher than that in soil from uncovered bamboo garden. With soil sterilization, conidium germination in the uncovered soil increased to the level of that in the covered bamboo garden soil. After addition of 6 antibiotics, there were significant difference in germination among the 6 antibiotics in the uncovered soil, but there were not difference in the covered soil. Results of soil 16 S rDNA metagenomics showed that both amount and species diversity of bacteria in the uncovered soil were higher than those in the covered soil, and bacteria of Acidobacteria, Actinobacteria and Gemmatimonadetes in the uncovered soil were significantly more abundant than those in the covered soil.