文献类型：期刊文献

英文题名：ADVENTITIOUS SHOOT REGENERATION FROM LEAF AND STEM EXPLANTS OF SALIX MATSUDANA KOIDZ

作者：Liu Xiaoxia[1] Sui Jinkai[1] Zhang Jianguo[1] Luo Ying[1] Rao Guodong[1]

通信作者：Rao, GD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：49

期号：2

起止页码：395-400

外文期刊名：BANGLADESH JOURNAL OF BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000572186600014)】；

基金：This work was supported by The Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2018QB001). The authors would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.

语种：英文

外文关键词：Adventitious shoot; Micropropagation; Regeneration; Salix matsudana

摘要：Currently, the leaf and stem as explants for rapid propagation in vitro remained unknown in Salix matsudana. Multiple shoots were regenerated from leaf and stem (stem apex and stem with axil). The optimum medium for callus regeneration and shoots induction from leaf was on WPM medium containing 6.0 mg/ l zeatin (Z) and 0.75 mg/l NAA. The optimum medium of shoot induction from stem apex was on MS medium supplemented with 8.0 mg/l Z and 1.0 mg/l NAA, and from stem with axil was on MS with 8.0 mg/ l Z and 0.5 mg/l NAA. Rooting of regenerated shoots was obtained on the same medium supplemented with 1.0 mg/ l activated charcoal.