文献类型：期刊文献

中文题名：灰木莲花药结构和花粉发育特征

英文题名：Anther Structure and Pollen Development Characteristic of Manglietia conifera

作者：潘丽琴[1] 郝建[2] 徐建民[3] 粟宏林[1] 梁福江[2] 张青青[1] 姜清彬[3]

第一作者：潘丽琴

机构：[1]西南林业大学,云南昆明650204;[2]中国林业科学研究院热带林业实验中心,广西凭祥532600;[3]中国林业科学研究院热带林业研究所,广东广州510520

年份：2021

卷号：34

期号：6

起止页码：107-113

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：广东省林业科技创新专项(2017KJCX027,2019KJCX012);中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016QA014)。

语种：中文

中文关键词：灰木莲;花药;花粉;小孢子;雄配子体

外文关键词：Manglietia conifera;anther;pollen;microspore;male gametophyte

分类号：S796

摘要：[目的]研究灰木莲花药结构及其小孢子发育过程以确定是否存在雄性不育的异常现象,并为进一步确立灰木莲分类学地位和木兰科系统发育的研究提供佐证和积累资料。[方法]通过解剖和形态观察对灰木莲的花药结构和小孢子及雄配子体发育过程进行观察。[结果]灰木莲成熟花药由表皮层、药室内壁、中层和绒毡层等5~7层细胞组成。4~6个小孢子囊呈线形排列,花药开裂方式为向心或侧向开裂。小孢子母细胞胞质分裂方式同时具有连续型和同时型2种类型。小孢子四分体排列方式为左右对称型、直线型或四面体型,成熟花粉二细胞型。花粉壁光滑,极轴面具极稀疏穴状纹饰。[结论]灰木莲花药结构和发育特征与其他木莲属相似,但在花粉囊结构、胞质分裂方式和小孢子四分体排列方式表现不同,小孢子发育过程的异常现象主要发生于四分体时期,为引起灰木莲花粉败育和结实率低的原因之一。
[Objective]The anther structure and microspore development of Manglietia conifera were studied to determine the abnormal phenomenon of male microspore development,and to provide evidence and accumulation data for further study on the taxonomic status of M.conifera and the phylogeny of Magnoliaceae.[Method]The anther structure,microspore and male gametophyte development of M.conifera were observed by means of anatomy and morphology.[Result]The results showed that the mature anther of M.conifera was composed of 5 to 7 layers of cells,including the surface layer,the inner wall,the middle layer and the tapetum.The 4-6 microsporangia were arranged in a linear pattern and the anther dehiscence was centripetal or lateral.There were two types of cytokinesis of microspore mother cell:continuous type and simultaneous type.The arrangement of microspore in a tetrad was isobilateral,linear or tetrahedral,and the mature pollen was two-celled.The pollen wall was smooth with very sparse cave-like ornamentation.[Conclusion]The anther structure and developmental characteristics of M.conifera were similar to those of other genera in Manglietia,but the structure of pollen sac,the mode of cytoplasm division and the arrangement of microspore tetrad were different.The abnormal phenomenon of microspore development mainly occurs in the tetrad stage,which is one of the reasons for the abortion the pollen and low seed setting rate of fruit.