文献类型：期刊文献

中文题名：油茶SRAP-PCR反应体系的优化

英文题名：Optimization of SRAP-PCR System for Camellia oleifera

作者：郑婷婷[1,2] 林萍[2] 王开良[2] 姚小华[2] 杨水平[1]

第一作者：郑婷婷

机构：[1]西南大学资源环境学院;[2]中国林业科学研究院亚热带林业研究所

年份：2010

期号：2

起止页码：302-307

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金："十一五"国家科技支撑专题"高产优质油茶香榧新品种选育"(2006BAD01A1706);中国林科院公益性科研基金专项"高油高抗油茶杂交新种质创制"(CAFYBB2008005);中国林科院亚林所公益性科研基金专项"油茶品种分子鉴别系统构建及油茶基因芯片开发"(RISF6804)

语种：中文

中文关键词：油茶;SRAP;正交设计;体系优化

外文关键词：Camellia oleifera ; SRAP; orthogonal design ; system optimization

分类号：S794.4

摘要：Camellia oleifera is one of the important oil tree species in south China,and C.oleifera industry is quickly developed with the support of the national policies in recent years.The disorder of C.oleifera varieties is one of the key issues restricting the development of C.oleifera industry.Because of high polymorphism,good repeatability,less use of DNA and so on,SRAP as a new marker was used in identification of cultivars,analysis of genetic resources and genetic diversity in recent years.In this paper,the orthogonal design was used to optimize SRAP-PCR system for C.oleifera by 5 factors(Mg2+,dNTPs,primer,Taq polymerase,DNA template) and 4 levels,respectively.The data were analyzed by software SPSS V13.0.A suitable SRAP-PCR system(20 μL) was established as: 75 ng DNA template,1.5 mmol·L-1 Mg2+,0.15 mmol·L-1 dNTPs,1U Taq DNA polymerase,0.4 μmol·L-1 primer,1×PCR buffer.The result of optimal SRAP-PCR system will provide a foundation for the identification of C.oleifera cultivars.
Camellia oleifera is one of the important oil tree species in south China, and C. oleifera industry is quickly developed with the support of the national policies in recent years. The disorder of C. oleifera varieties is one of the key issues restricting the development of C. oleifera industry. Because of high polymorphism, good repeatability, less use of DNA and so on, SRAP as a new marker was used in identification of cuhivars, analysis of genetic resources and genetic diversity in recent years. In this paper, the orthogonal design was used to optimize SRAP-PCR system for C. oleifera by 5 factors （ Mg^2＋ , dNTPs, primer, Taq polymerase, DNA template） and 4 levels, respectively. The data were analyzed by software SPSS V13.0. A suitable SRAP-PCR system （20μL） was established as： 75 ng DNA template, 1.5 mmol· L^-1 Mg2＋ , 0.15 mmol · L^-1 dNTPs, 1U Taq DNA polymerase, 0.4 mmol· L^-1primer, 1 × PCR buffer. The result of optimal SRAP-PCR system will provide a foundation for the identification of C. oleifera cuhivars.